不同种植模式下凤丹根际微生物群落结构和多样性分析  

Analysis on community structure and diversity in rhizosphere microorganisms of Paeonia ostii under different planting patterns

作　　者：刘智 张虹 罗会婷[1] 王仲伟 汤诗杰[1] LIU Zhi;ZHANG Hong;LUO Hui-ting;WANG Zhong-wei;TANG Shi-jie(Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,China;Graduate School,Nanjing University of Chinese Medicine,Nanjing 210046,China)

机构地区：[1]江苏省中国科学院植物研究所,江苏南京210014 [2]南京中医药大学研究生院,江苏南京210046

出　　处：《江苏农业学报》2022年第2期502-511,共10页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省农业科技自主创新基金项目[CX(19)3066];江苏现代农业产业技术体系建设项目[JATS(2021)507]。

摘　　要：为探究不同种植模式下凤丹根际微生物群落结构和多样性,采用高通量测序技术对采自榉树(Zelkova serrata)下种植(ZS)、娜塔栎(Quercus nattallii)下种植(QS)、露天种植(OS)的凤丹根际以及空白土壤(CK)样品土壤细菌和真菌进行测序。结果显示,与空白土壤相比,种植凤丹提高了根际微生物群落的丰富度和多样性,不同种植模式下凤丹根际微生物群落结构有所差异。3种种植模式下细菌的优势菌门均为变形菌门(Proteobacteria)、酸杆菌门(Acidobacteria)、放线菌门(Actinobacteria)以及疣微菌门(Verrucomicrobia),但不同种植模式下每个门的相对丰度有所差异。所有样品中总共检测出12个真菌门,3种种植模式下真菌的优势菌门均为子囊菌门(Ascomycota)和担子菌门(Basidiomycota),不同种植模式下各真菌门的相对丰度存在差异;属水平上真菌群落结构差异较大,ZS组、QS组和OS组的优势菌属分别为Exophiala、Thelephoraceae_unclassified、Ascomycota_unclassified。测定3种种植模式下凤丹的生长指标,发现林下种植的凤丹株高、叶长、叶宽均高于露天种植。林下种植模式的细菌需氧表型、革兰氏阴性表型、生物膜形成表型以及致病潜力表型高于单一种植模式。本研究从根际微生物角度分析了不同种植模式对凤丹的影响,为凤丹林下种植提供参考,为后续促生菌筛选、菌肥研制提供理论支撑。In order to explore the rhizosphere microbial community structure and diversity of Paeonia ostii under different planting patterns,high-throughput sequencing technology was used to sequence the rhizosphere soil samples of Paeonia ostii collected from three different planting patterns,namely Zelkova serrata(ZS),Quercus nattallii(QS)and open-air planting(OS)and blank soil(CK).The results showed that compared with the blank soil,planting Paeonia ostii increased the richness and diversity of rhizosphere microbial community,and the rhizosphere microbial community structure of Paeonia ostii was different under different planting patterns.The dominant phyla of bacteria under three planting patterns were Proteobacteria,Acidobacteria,Actinobacteria and Verrucomicrobia,but the relative abundance of each phylum under different planting patterns was different.A total of 12 fungal phyla were detected in all samples.The dominant phyla of fungi under three planting modes were Ascomycota and Basidiomycota,and the relative abundance of each fungal phylum was different under different planting patterns.The fungal community structure was quite different at genus level,and the dominant genera in ZS group,QS group and OS group were Exophiala,Thelephoraceae_unclassified and Ascomycota_unclassified,respectively.The growth indices of Paeonia ostii under three planting patterns were measured,plant height,leaf length and leaf width of Paeonia ostii planted under forest were higher than those planted in open field.The aerobic phenotype,Gram-negative phenotype,forms biofilms phenotype and potentially pathogenic phenotype in cultivation pattrerns under forest were higher than those in single planting mode.In this study,the effects of different planting patterns on Paeonia ostii were analyzed from the perspective of rhizosphere microorganisms,so as to provide reference for understory planting of Paeonia ostii,and provide theoretical support for subsequent screening of probiotics and development of bacterial fertilizer.

关 键 词：凤丹 根际微生物 高通量测序 微生物多样性 微生物群落结构 

分 类 号：S154.3[农业科学—土壤学]