LC-MS/MS检测豆芽中的4种植物生长调节剂  

Determination of 4 plant growth regulators in bean sprouts by liquid chromatography-tandem mass sepectrumetry

作　　者：黄景怡 郭凯 王宗义 闫珺 黄丁偎 董子琦 张嘉彤 魏朦 HUANG Jingyi;GUO Kai;WANG Zongyi;YAN Jun;HUANG Dingwei;DONG Ziqi;ZHANG Jiatong;WEI Meng(Beijing Laboratory of Food Quality and Safety,Beijing Key Laboratory of Agricultural Product Detection and Control for Spoilage Organisms and Pesticides,College of Food Sciences and Engineering,Beijing University of Agriculture,Beijing 102206,China)

机构地区：[1]北京农学院食品科学与工程学院/食品质量与安全北京实验室/农产品有害微生物及农残安全检测与控制北京市重点实验室,北京102206

出　　处：《北京农学院学报》2022年第2期117-120,共4页Journal of Beijing University of Agriculture

基　　金：北京农学院大学生科研训练项目;全国大学生生物竞赛参赛项目。

摘　　要：【目的】建立豆芽中4-氯苯氧乙酸(4-CPA)、6-苄基腺嘌呤(6-BAP)、赤霉素(GAs)和2,4-滴(2,4-D)等4种典型植物生长调节剂(PGRs)的高效液相色谱-串联质谱(LC-MS/MS)检测方法。【方法】豆芽样品经均质、乙腈提取、盐析和稀释后,液相色谱-串联质谱(LC-MS/MS)多反应监测(MRM)模式检测,基质标准曲线法定量。【结果】检测方法线性良好,R;>0.995,检出限(LOD)为0.07~5.62μg/kg,定量限为0.25~18.74μg/kg,加标回收率为73.81%~109.57%,相对标准偏差为3.71%~16.88%;对50个样本的实际检测显示,6-BAP检出率为88%,但含量小于LOQ,其他PGRs未检出。【结论】该方法样品处理简单,能够满足豆芽中4种典型PGRs监测的需要。[Objective]To establish a improved method for determination of 4-chlorophenoxyacetic acid(4-CPA), 6-benzyl adenine(6-BAP), gibberellin(GAs) and 2,4-drops(2,4-D) in bean sprouts by liquid chromatography-tandem mass spectrometry(LC-MS/MS).[Method] With homogenizing, extracting with acetonitrile, salting out and diluting, the 4 PGRs in the samples extracts were determined by liquid chromatography-tandem mass spectrometry(LC-MS/MS) in multiple reaction monitoring(MRM) mode, and quantified by external standard method with matrix standards curves, and total 50 samples from makets were tested with the method.[Results]The method established was linearity with R;>0.995;The limit of detection(LOD) is 0.07-5.62 μg/kg;the limit of quantification(LOQ) is 0.25-18.74 μg/kg;the recoveries were 73.81%-109.57% with the relative standard deviation rang of 3.71%-16.88%;the determining results showed that the detection rate of 6-BAP was 88%, but it contents were less than LOQ, and other PGRs were not detected.[Conclusion]The established method was simple with the sample pretreatment and could meet the requirements for monitoring 4 typical PGRs in bean sprouts.

关 键 词：豆芽 植物生长调节剂 液相色谱-串联质谱 

分 类 号：TS227[轻工技术与工程—粮食、油脂及植物蛋白工程]