芝麻子粒脂肪酸主要组分的近红外光谱模型建立及特征分析  

Establishment of Near Infrared Spectral Model and Characteristic Analysis of Main Components of Fatty Acids in Sesame Seeds

作　　者：袁青丽 苗红梅[1] 马琴[1] 琚铭[1] 张欣童 张海洋[1] YUANG Qing-li;MIAO Hong-mei;MA Qin;JU Ming;ZHANG Xin-tong;ZHANG Hai-yang(Henan Key Laboratory of Specific Oilseed Crops Genomics/Henan Sesame Research Center,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China)

机构地区：[1]河南省特色油料作物基因组学重点实验室/河南省农业科学院芝麻研究中心,河南郑州450002

出　　处：《河北农业科学》2022年第2期102-108,共7页Journal of Hebei Agricultural Sciences

基　　金：国家现代农业(特色油料)产业技术体系建设项目(CARS-14);河南省重大科技专项(201300110600);河南省科技创新领军人才项目(214200510020);河南中原科学家工作室建设项目(214400510026);河南省农业科学院科技创新创意项目(2020CX02);河南省农业科学院优秀青年项目(2020YQ19);河南省农业科学院自主创新项目(2021ZC70)。

摘　　要：为准确建立芝麻子粒脂肪酸组分的近红外光谱(NIRS)模型,实现子粒品质的快速、精准检测,选用116份代表性黄白芝麻种质,分别采用气相色谱法(国标法)和NIRS法,测定了成熟芝麻子粒的油酸、亚油酸、棕榈酸和硬脂酸含量;并采用改进偏最小偏二乘法技术(MPLS),成功建立了定标模型以及上述指标的NIRS模型。结果表明:芝麻子粒油酸、亚油酸、棕榈酸、硬脂酸含量的定标决定系数(RSQ)分别为0.981、0.986、0.898和0.780,标准偏差/交互标准偏差(SEC/SECV)分别为0.648、0.578、0.587和0.728;绝对误差均控制在1.5%以下。该模型可稳定反映出黄白芝麻种质的脂肪酸组分含量,为加快芝麻品质遗传研究提供了可靠技术。In order to accurately establish the near infrared spectroscopy(NIRS)model of fatty acid components in sesame seeds and realize the rapid and accurate detection of seed quality,116 representative yellow and white sesame germplasm were selected.The contents of oil acid,linoleic acid,palmitic acid and stearic acid in mature sesame seeds were determined by gas chromatography(national standard method)and NIRS respectively.The calibration model and NIRS model of the above indexes were successfully established by using the improved partial least partial square(MPLS)technology.The results showed that the RSQ of oleic acid,linoleic acid,palmitic acid and stearic acid in sesame seeds was 0.981,0.986,0.898 and 0.780 respectively,and the standard deviation/interactive standard deviation(SEC/SECV)was 0.648,0.578,0.587 and 0.728 respectively.The absolute error was controlled below 1.5%.The model could stably reflect the fatty acid content of yellow and white sesame germplasm,and provide a reliable technology for accelerating the genetic research of sesame quality.

关 键 词：芝麻 脂肪酸组分 近红外光谱 定标模型 

分 类 号：S565.3[农业科学—作物学]