长链非编码RNA(LncRNA)在印度梨形孢促进大麦根部生长发育中的调控作用  

Regulation of long non-coding RNA(LncRNA)in barley roots in response to Piriformospora indica colonization

作　　者：郭楠楠 刘天策 史硕 胡心亭 牛亚丹 李亮[1] GUO Nan-Nan;LIU Tian-Ce;SHI Shuo;HU Xin-Ting;NIU Ya-Dan;LI Liang(College of Chemical Engineering and Technology,Hebei University of Technology,Tianjin 300130,China)

机构地区：[1]河北工业大学化工学院,天津300130

出　　处：《作物学报》2022年第7期1625-1634,共10页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31801948);河北省重点研发计划项目(19226505D);河北省自然科学基金项目(C2021202005)资助。

摘　　要：印度梨形孢定殖植物会促进植物生物产量提高,其分子机制有待深入挖掘。LncRNA是一类长链非编码RNA,在植物生长发育过程中具有重要调控作用。然而,目前我们还不清楚大麦中LncRNA是否对印度梨形孢的定殖有响应。本研究发现,印度梨形孢定殖大麦会诱导大麦根系迅速发育,促进较多根分枝。采用全基因组高通量测序RNA-seq和生物信息学方法鉴定LncRNA,发现在P.indica定殖后3 d和7 d分别有752个和932个差异表达的LncRNA,7 d相对于3 d有70个差异表达的LncRNA。其中在P.indica定殖后3 d有375个LncRNA表达上调,377个LncRNA表达下调;在P.indica定殖后7 d有459个LncRNA表达上调,473个LncRNA表达下调;7 d相对于3 d组中,有39个LncRNA表达上调,31个LncRNA表达下调。qPCR验证LncRNA的表达与RNA-seq结果一致。GO和KEGG分析表明,在P.indica定殖大麦促生过程中,部分LncRNA参与了激素信号途径的转录调控。该工作对于进一步理解LncRNA与靶基因的相互作用以及其对靶基因的调控功能提供了新的理论基础和实验依据,并以LncRNA为靶点,进行作物性状改良提供新的思路和方向。The molecular mechanism of biomass enhancement by Piriformospora indica in colonization plants needs to be further explored.LncRNA is a kind of long-chain non-coding RNA,which plays an important role in the regulation of plant growth and development.However,it remains unclear whether barley LncRNAs are responsive to Piriformospora indica colonization.It was found that barley roots exhibited fast development and large roots branched after P.indica colonization.Genome-wide high throughput RNA-seq and bioinformatical analysis showed that 752 and 932 differentially expressed LncRNAs were detected in responsive to P.indica at 3-day and 7-day after colonization,respectively.And 70 differentially expressed LncRNAs were found at 7-day compared to 3-day.Among these,375 were up-regulated and 377 were down-regulated after 3 days’colonization,and 459 were up-regulated and 473 were down-regulated after 7 days’colonization,39 were up-regulated and 31 were down-regulated in 7-day to 3-day comparison group.The qPCR results verified the validity of LncRNAs data in RNA-seq.GO and KEGG analy-sis indicated that a few LncRNAs might be involved in the molecular functions,cellular components,and biological processes upon P.indica colonization.This study provides a new theoretical basis and experimental basis for further understanding of the interaction between LncRNAs and coding sequences and regulatory functional networks,and provides new ideas and directions for crop shape improvement based on LncRNAs.

关 键 词：LncRNA 大麦 印度梨形孢 RNA-SEQ 转录因子 细胞周期 

分 类 号：S512.3[农业科学—作物学]