经TACE治疗的肝细胞癌患者外周血单个核细胞中沉默信息调节因子7的表达及其与预后的关系  

Relationship between expression of silent information regulator 7 in peripheral blood mononuclear cells after TACE treatment of hepatocellular carcinoma and prognosis of patients

作　　者：卢金兰 王丽丽 卢哲 LU Jinlan;WANG Lili;LU Zhe(Department of Laboratory and Pathology,Beijing Armed Police Corps Hospital,Beijing 100027,China;Department of Clinical Laboratory,Sixth Medical Center of PLA General Hospital,Beijing 100048,China)

机构地区：[1]武警北京市总队医院检验与病理科,100027 [2]中国人民解放军总医院第六医学中心检验科,北京100048

出　　处：《国际消化病杂志》2022年第2期105-109,共5页International Journal of Digestive Diseases

摘　　要：目的检测肝细胞癌(HCC)患者接受经导管动脉化学治疗栓塞术(TACE)治疗后外周血单个核细胞中沉默信息调节因子7(SIRT7)的表达水平,并分析其与患者预后的关系。方法选择2016年8月至2018年3月在武警北京市总队医院就诊的104例HCC患者作为研究对象。根据患者预后3年生存情况将其分为生存组(n=71)和死亡组(n=33)。采用Ficoll密度梯度离心法提取外周血中的单个核细胞。采用蛋白质印迹法检测单个核细胞中SIRT7的表达水平。采用ROC曲线评估SIRT7判断经TACE治疗的HCC患者预后的效能。采用Cox比例风险回归模型分析经TACE治疗的HCC患者预后的影响因素。结果与治疗前比较,生存组和死亡组治疗后SIRT7的表达水平均较低,差异均有统计学意义(P均<0.05)。与死亡组比较,生存组治疗前和治疗后SIRT7的表达水平均较低,差异均有统计学意义(P均<0.05)。治疗后SIRT7水平判断HCC患者预后生存的ROC曲线下面积(AUC)大于治疗前SIRT7水平及治疗前后SIRT7水平的差值(P均<0.05)。Cox比例风险回归模型分析结果显示,肿瘤分期、肿瘤直径和治疗后SIRT7是经TACE治疗的HCC患者预后生存的独立危险因素(P<0.05),索拉非尼和利卡汀是其独立保护因素(P<0.05)。结论经TACE治疗的HCC患者外周血单个核细胞中SIRT7的表达水平与患者预后生存情况有关。Objective This paper aims to detect the expression level of silent information regulator 7(SIRT7)in peripheral blood mononuclear cells of patients with hepatocellular carcinoma(HCC)after transcatheter arterial chemoembolization(TACE),and to analyze its relationship with the prognosis of patients.Methods A total of 104 HCC patients who were treated in Beijing Armed Police Corps Hospital from August 2016 to March 2018 were selected and divided into the survival group(n=71)and the death group(n=33)according to their prognosis and 3-year survival.Mononuclear cells from peripheral blood were extracted by Ficoll density gradient centrifugation.The expression level of SIRT7 in mononuclear cells was detected by Western blotting.The ROC curve was used to evaluate the efficacy of SIRT7 in diagnosing the prognosis of TACE-treated HCC patients.The Cox proportional hazards regression model was used to analyze the prognostic factors of HCC patients treated with TACE.Results Compared with the expression levels of SIRT7 before treatment,those after treatment in the survival group and the death group are lower,with statistically significant differences(P<0.05).Compared with the death group,the expression levels of SIRT7 before and after treatment in the survival group are lower,with statistically significant differences(P<0.05).The area under the ROC curve(AUC)of the SIRT7 after treatment to diagnose the prognosis of HCC patients is greater than that of the pre-treatment SIRT7 and that of the post-treatment SIRT7(P<0.05).The results of Cox proportional hazards regression model analysis show that tumor stage,tumor diameter,and SIRT7 after treatment are independent risk factors for the prognosis of HCC patients treated with TACE(P<0.05),and sorafenib and licatin are independent protective factors(P<0.05).Conclusion The expression level of SIRT7 in peripheral blood mononuclear cells of HCC patients treated with TACE is related to the prognosis of patients.

关 键 词：肝细胞癌 经导管动脉化学治疗栓塞术 外周血单个核细胞 沉默信息调节因子7 预后 

分 类 号：R735.7[医药卫生—肿瘤]