Preliminary study on the safety and efficacy of a new polymyxin B-immoblized resin column in treatment of LPS-induced sepsis beagles  

作　　者：Yonggui Li Zhenggen Yang Jialiang Hu Zhennan Lin 

机构地区：[1]Research and Development Center,Guangzhou Koncen Bioscience Co.,Ltd.,Guangzhou,PR China [2]Guangdong Provincial Key Laboratory of Hemoadsorption Technology,Guangzhou,PR China

出　　处：《Animal Models and Experimental Medicine》2022年第2期161-171,共11页动物模型与实验医学（英文）

基　　金：This research was financially supported by Guangdong Provincial Key Laboratory of Hemoadsorption Technology(No:2020B121202021)。

摘　　要：Background:This study aims to assess the safety and efficacy of direct hemoperfu-sion using a new polymyxin B-immobilized resin column(disposable endotoxin ad-sorber,KCEA)in an endotoxin/lipopolysaccharide(LPS)-induced sepsis model.Methods:Eighteen beagles were randomized into 1 intervention group(KCEA group,n=6)and 2 control groups(sham group and model group,n=6 each).Sepsis was in-duced by continuous intravenous application of 0.5 mg/kg body weight of endotoxin for 60 min.An extracorporeal hemoperfusion device made with KCEA for endotoxin adsorption was used.Model group beagles received standard treatment with fluids and vasoactive drugs,KCEA group beagles received standard treatment and direct hemoperfusion of KCEA for 2 h,and sham group beagles were treated with standard treatment and direct hemoperfusion of a sham column for 2 h.Results:Good blood compatibility of KCEA was confirmed by assessing clinical pa-rameters.Blood endotoxin peak levels in the KCEA group were significantly lower,resulting in a significant suppression of IL-6,TNF-αand procalcitonin,which improved mean arterial pressure and significantly lowered vasopressor demand,thereby pro-tecting organ function and improving survival time and rate.In the KCEA group,MAP was significantly higher over 6 h than those recorded both in the sham group and model group.The 7-day survival rates of the KCEA,sham and model groups were 50%,0%and 0%,respectively.Conclusion:KCEA hemoadsorption was effective at detoxifying circulatory endotoxin and inflammatory mediators and contributed to the decreased mortality rate in the sepsis beagles.

关 键 词：ENDOTOXIN hemoadsorption polymyxin B sulfate RESIN SEPSIS 

分 类 号：R459.7[医药卫生—急诊医学]