超高效液相色谱串联质谱法鉴定皮革的动物源属性  

Identification of the Animal Origins of Leather by UPLC-MS/MS

作　　者：杜业刚[1] 刘奕雄[1] 王韦达[1] 朱雨田[1] 郑燕燕[1] DU Yegang;LIU Yixiong;WANG Weida;ZHU Yutian;ZHENG Yanyan(Shenzhen Academy of Metrology and Quality Inspection,National Nutrition Food Testing Center(Guangdong),Shenzhen 518102,China)

机构地区：[1]深圳市计量质量检测研究院,国家营养食品质量检验检测中心(广东),广东深圳518102

出　　处：《皮革科学与工程》2022年第3期74-78,共5页Leather Science and Engineering

基　　金：深圳市科技计划项目(YCYJ20170817162416978)。

摘　　要：基于猪牛羊皮的特征肽段建立了一种鉴定皮革动物源属性的超高效液相色谱串联质谱法(UPLC-MS/MS)。皮革样品经高温高压提取蛋白,酶解消化,收集多肽待测溶液,经C_(18)柱分离,以0.1%甲酸水溶液和乙腈为流动相梯度洗脱,通过多反应监测(MRM)扫描,从多肽溶液中特征肽段的出峰情况来鉴定皮革样品的动物源属性。所建立的方法特异性强,适用范围广,准确可靠,为皮革鉴定提供了新的技术手段。An ultra high performance liquid chromatography tandem-mass spectrometry(UPLC-MS/MS)method was developed for the identification of animal origins for leather based on the peptide markers of porcine,bovine and sheep skins.Proteins were firstly extracted from the leather samples under high temperature and high pressure,and then digested by enzymolysis.The peptide solutions were separated by C_(18)column and eluted with the aqueous solution of formic acid and acetonitrile as mobile phases.The animal origins of the leather samples were identified by the peaks of peptide markers in the peptide solution by multiple reaction monitoring(MRM)scanning.The established method has strong specificity,wide application range,good accurateness and reliablity,and provides a new technical means for leather identification.

关 键 词：皮革 液相色谱-串联质谱 特征肽段 

分 类 号：O657.63[理学—分析化学] TS57[理学—化学]