农村生活污水贝类净化区细菌群落结构解析  

Analysis of Bacterial Community Structure in Shellfish Purification Area of Rural Domestic Sewage

作　　者：高娜 梁阳阳 李静[1] 杨坤[1] 方婷[1] 赵秀侠[1] 崔凯[1] 卢文轩[1] GAO Na;LIANG Yang-yang;LI Jing(Fisheries Research Institute,Anhui Academy of Agricultural Sciences,Key Laboratory of Freshwater Aquaculture and Enhancement of Anhui Province,Hefei,Anhui 230000)

机构地区：[1]安徽省农业科学院水产研究所水产增养殖安徽省重点实验室,安徽合肥230000

出　　处：《安徽农业科学》2022年第9期83-86,共4页Journal of Anhui Agricultural Sciences

基　　金：安徽省农业科学院科技创新团队项目(2021YL055);安徽省中央引导地方科技发展专项(201907d06020003);国家现代农业产业技术体系专项(CARS-46);安徽省水产产业技术体系(皖农科函[2021]711号)。

摘　　要：[目的]研究农村生活污水贝类净化区的细菌群落结构。[方法]采用Illumina-MiSeq高通量测序技术对贝类处理单元水体及贝类样品进行细菌多样性分析。[结果]贝类样品中的细菌群落多样性和丰度均显著高于处理单元水体。4组样品中均有大量序列不能被归入已知的属,在门的分类水平上,优势菌门是蓝细菌门(Cyanobacteria)和放线菌门(Actinobacteriota);在属水平上,优势细菌属主要为Cyanobium_PCC_6307、hgc I_clade。菌群分析发现贝类处理单元水体可能处于富营养化进程中。水质检测分析发现贝类通过滤食作用对氨氮、总磷和COD的去除率分别达到39.75%、37.21%、59.52%。[结论]该研究为贝类净水技术提供基础数据,对贝类处理农村生活污水系统的构建具有指导意义。[Objective]To study the microbial community composition in shellfish purification area of rural domestic sewage.[Method]The Illumina-MiSeq high-throughput sequencing technology was used to analyze bacterial diversity in shellfish treatment unit water and shellfish samples. [Result]The diversity and abundance of bacterial community in the shellfish samples were significantly higher than those in the water of the treatment unit.There were a large number of sequences in the four samples that cannot be classified into known genera.The dominant bacteria were Cyanobacteria and Actinobacteriota at phylum level. At the genus level, the mainly bacteria are Cyanobium-PCC-6307 and hgc Iclade. Microbiota analysis showed that the water of the shellfish treatment unit may be in the process of eutrophication. Water quality detection and analysis showed that the removal rates of ammonia nitrogen, total phosphorus and COD by shellfish filter-feeding were 39.75%, 37.21% and 59.52%, respectively. [Conclusion]The research provides a theoretical basis for shellfish water purification technology, and it has guiding significance to the construction of shellfish treatment rural sewage system.

关 键 词：农村生活污水 淡水贝类 净化区 高通量测序 群落结构 细菌多样性 

分 类 号：X703[环境科学与工程—环境工程]