中药药敏试验新方法及68种中药对克氏原螯虾病原菌的抑菌活性研究  

Study on A New Method for Drug Susceptibility Test of Traditional Chinese Medicine and Antibacterial Activity of 68 Kinds of Traditional Chinese Medicine against Pathogenic Bacteria of Procambarus clarkii

作　　者：邹文腾 彭开松[1] 张子涵 奚业文[2] 梅正龙 ZOU Wen-teng;PENG Kai-song;ZHANG Zi-han(Laboratory of Aquatic Health and Public Health,Department of Fisheries,Col-lege of Animal Science and Technology,Anhui Agricultural University,Hefei,Anhui 230036)

机构地区：[1]安徽农业大学动物科技学院水产系水生健康与公共卫生实验室,安徽合肥230036 [2]安徽省水产技术推广总站,安徽合肥230601 [3]安徽省定远县水务局,安徽定远233200

出　　处：《安徽农业科学》2022年第9期87-90,95,共5页Journal of Anhui Agricultural Sciences

基　　金：安徽省现代农业产业技术体系项目(皖农科[2016]84号)。

摘　　要：为消除中药颜色和不溶颗粒对最小抑菌浓度测定结果判定的影响,建立了微量肉汤稀释联合平板计数法。结果发现,抗菌剂(磺胺甲噁唑、多西环素、氟苯尼考、硫酸新霉素、恩诺沙星)MIC测定前后的MIC孔细菌浓度比值(BCR_(MIC))为10^(-3)~10^(3)。以BCR_(MIC)为标准替代肉眼观察来判定中药MIC,采用微量肉汤稀释联合平板计数法定量评价了68种中药的抗菌活性。五倍子、黄连、山楂、地锦草、厚朴、黄芩、石榴皮、翻白草、乌梅对克氏原螯虾源病原菌(嗜水气单胞菌、维氏气单胞菌、弗氏柠檬酸杆菌)的抗菌活性较强,其MIC为4~16 mg/mL。In order to eliminate the influences of the color and insoluble particles of traditional Chinese Medicine on the determination of minimal inhibitory concentration(MIC) of traditional Chinese medicine, the micro broth dilution combined with plate counting method(MBD&PCM) was established. The results showed that the bacterial concentration ratio(BCR_(MIC)) in MIC cell before and after MIC determination of antibiotics(sulfamethoxazole, doxycycline, florfenicol, neomycin sulfate, enrofloxacin) was between 10^(-3)and 10^(3). The antibacterial activity of 68 kinds of traditional Chinese medicines was quantitatively evaluated by MBD&PCM, and BCR_(MIC)was used as the standard instead of naked eye observation to determine MIC of traditional Chinese medicine. The antibacterial activity of 68 kinds of traditional Chinese medicine was quantitatively evaluated by MBD&PCM. Galla chinensis, Coptids rhizoma, Crataegi fructus, Euphorbia humifusae herba, Magnolia officinalis cortex, Scutellariae radix, Cranati pericarpium, Potentillae discoloris herba and Mume fructus had strong antibacterial activities against pathogenic bacteria(Aeromonas hydrophila, Aeromonas veronica and Citrobacter freundii) of Procambarus clarkii.

关 键 词：中药 最小抑菌浓度 大肠杆菌 气单胞菌 柠檬酸杆菌 克氏原螯虾 

分 类 号：S945.4[农业科学—水产养殖]