上调微小RNA-520a对胃癌细胞恶性生物学行为和放射增敏性的影响及相关机制  

Effect and related mechanism of microRNA-520 a up-regulation on malignant biological behaviors and enhanced radiosensitivity in gastric cancer cells

作　　者：杨慧 李相勇 YANG Hui;LI Xiang-yong(Department of Hematology and Oncology Ward 2,the 904 Hospital of the Joint Logistics Support Force of the Chinese People′s Liberation Army,Wuxi 214000,China)

机构地区：[1]中国人民解放军联勤保障部队第九〇四医院血液肿瘤科二区,江苏省无锡市214000

出　　处：《广西医学》2022年第4期407-411,429,共6页Guangxi Medical Journal

基　　金：江苏省南京军区医药卫生科研基金(MS013)。

摘　　要：目的探讨上调微小RNA-520a(miR-520a)对胃癌细胞恶性生物学行为和放射增敏性的影响及相关机制。方法将胃癌MGC-803细胞分为空白组、上调组、下调组,空白组加入常规细胞培养液,下调组给予转染p-Genesil,上调组给予转染p-Genesil-miR-520a。鉴定各组细胞转染情况,并检测细胞增殖、凋亡、迁移、侵袭能力以及Janus激酶3(JAK3)/信号转导及转录激活因子3(STAT3)信号通路蛋白表达量。各组细胞在转染结束后,接受一定剂量的X线照射,观察细胞集落形成情况,并计算平均致死量(D0)、2 Gy剂量X线照射下的细胞存活分数(SF2)和放射增敏比(SER)。结果转染48 h后,上调组miR-520a表达量高于空白组,下调组miR-520a表达量低于空白组及上调组(均P<0.05)。稳定转染后24 h、48 h、72 h,上调组细胞增殖率低于空白组及下调组,下调组细胞增殖率高于空白组;72 h后,上调组及下调组细胞凋亡率均高于空白组,且上调组高于下调组(均P<0.05)。上调组细胞迁移、侵袭数少于空白组及下调组,但下调组细胞迁移、侵袭数多于空白组(均P<0.05)。上调组SF2值、D0值和SER大于空白组及下调组,下调组SF2值、D0值和SER小于空白组(均P<0.05)。上调组JAK3、STAT3、磷酸化JAK3、磷酸化STAT3蛋白表达量均低于空白组及下调组,下调组JAK3、STAT3、磷酸化JAK3、磷酸化STAT3蛋白表达量均高于空白组(均P<0.05)。结论上调miR-520a表达水平可以抑制胃癌细胞增殖、迁移、侵袭,促进细胞凋亡,并可增强细胞的放射增敏性,其可能通过影响JAK3/STAT3信号通路的表达发挥作用。Objective To explore the effect and related mechanisms of microRNA-520a(miR-520a)up-regulation on malignant biological behaviors and enhanced radiosensitivity in gastric cancer cells.Methods Gastric cancer MGC-803 cells were divided into blank group,up-regulation group and down-regulation group.The blank group was added with conventional cell culture medium,the down-regulation group was transfected with p-Genesil,and the up-regulation group was transfected with p-Genesil-miR-520a.The conditions of cell transfection were checked in each group;besides,the abilities of cell proliferation,apoptosis,migration,and invasion,as well as the protein expression of Janus kinase 3(JAK3)/signal transducer and activator of transcription 3(STAT3)signaling pathway,were detected in each group.After the end of the transfection,cells in each group received an X-ray irradiation at certain doses,and the status of cell colony forming was observed;in addition,the mean fatality dose(D0),cell survival fraction under the X-ray irradiation at a dose of 2 Gy(SF2)and radiosensitivity enhancement ratio(SER)were calculated.Results After 48 hours of the transfection,the up-regulation group exhibited higher miR-520a expression than the blank group,and the down-regulation group exhibited lower miR-520a expression than the blank and up-regulation groups(all P<0.05).Twenty-four,48 and 72 hours after the stable transfection,the up-regulation group reported lower rates of cell proliferation than the blank and down-regulation groups,and the down-regulation group reported higher rates of cell proliferation than the blank group;after 72 hours,the up-regulation and down-regulation groups exhibited higher apoptosis rates than the blank group,in terms of which the up-regulation group was higher than the down-regulation group(all P<0.05).The numbers of cell migration and invasion were fewer in the up-regulation group than in the blank and down-regulation groups,but the numbers of cell migration and invasion in the down-regulation group were more than those i

关 键 词：胃癌 微小RNA-520a 恶性生物学行为 放射增敏 Janus激酶3/信号转导及转录激活因子3信号通路 细胞实验 

分 类 号：R735.2[医药卫生—肿瘤]