长期酒精摄入对小鼠小脑苔藓纤维-颗粒细胞突触可塑性及运动协调功能的影响  

Effects of long-term alcohol consumption on synaptic plasticity of mossy fiber-granule cells in cerebellar cortex and motor coordination function in mice

作　　者：董光辉 李文静 邱德来[3] 崔松彪 Dong Guanghui;Li Wenjing;Qiu Delai;Cui Songbiao(Department of Neurology,Affiliated Hospital of Yanbian University,Yanji 133000,China;Department of Anesthesiology,Affiliated Hospital of Yanbian University,Yanji 133000,China;Brain Science Research Center of Yanbian University,Yanji 133000,China)

机构地区：[1]延边大学附属医院神经内科,延吉133000 [2]延边大学附属医院麻醉科,延吉133000 [3]延边大学脑科学研究中心,延吉133000

出　　处：《中华行为医学与脑科学杂志》2022年第3期205-211,共7页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金(81860219)。

摘　　要：目的探究长期酒精摄入对小鼠小脑皮层苔藓纤维-颗粒细胞突触可塑性及运动功能的影响。方法 30只清洁级6~8周龄健康ICR小鼠(性别不限)按照随机数字表法分为生理盐水组(对照组)和酒精摄入组(酒精组),每组15只,酒精组小鼠每日腹腔注射浓度为15%乙醇(1.6 g/kg),对照组小鼠则注射同等剂量的生理盐水,1次/d,连续注射28 d。使用行走障碍实验和转棒疲劳实验观察小鼠的运动协调能力和学习能力;电生理膜片钳技术检测吹风刺激诱发的长时程突触可塑性的场电位变化。采用SPSS 22.0软件进行统计分析,独立样本t检验、配对t检验和重复测量方差分析进行两组间及干预前后的比较。结果电生理结果显示,吹风刺激后,对照组小鼠场电位N1波的振幅百分比[(130.4±3.3)%]高于刺激前[(100.6±2.7)%](t=27.07,P<0.01);刺激后N1波的波形下面积百分比[(128.8±4.5)%]较刺激前[(100.2±3.5)%]高(t=19.43,P<0.01)。吹风刺激后,酒精组小鼠N1波的振幅百分比[(97.8±4.3)%],与刺激前[(99.5±5.6)%]相比差异无统计学意义(t=0.93,P>0.05);刺激后N1波的波形下面积百分比[(96.8±3.6)%]与刺激前[(100.2±4.2)%]相比差异无统计学意义(t=2.38,P>0.05)。行走障碍实验结果显示,酒精组小鼠错误总数[(3.14±0.19)次]较对照组[(1.52±0.29)次]高(t=17.87,P<0.01),总错误时间[(63.85±9.34) ms]较对照组[(28.93±7.21) ms]长(t=11.45,P<0.01)。两组小鼠转棒疲劳实验数据使用重复测验方差分析,结果显示两组小鼠的跌落速度和跌落潜伏期均存在时间和组别的交互作用(F=4.5,455.1,均P<0.05)。酒精组小鼠第1~7天跌落速度明显低于对照组,均差异有统计学意义(均P<0.05);酒精组小鼠第1~7天跌落潜伏期均短于对照组,均差异有统计学意义(均P<0.05)。结论长期酒精摄入损害小鼠颗粒层突触可塑性并导致小鼠运动协调功能及运动学习能力明显下降。Objective To investigate the long-term alcohol consumption on synaptic plasticity of mossy fiber-granule cells in cerebellar cortex and motor coordination function in mice.Methods Thirty healthy male ICR mice aged 6-8 weeks were divided into saline group(control group)and alcohol consumption group(alcohol group)according to random number table with 15 in each group.The mice in alcohol group were injected intraperitoneally with 15%ethanol(1.6 g/kg),while the mice in control group were injected with the same volum of normal saline,all mice were injected intraperitoneally once a day for 28 consecutive days.Walking obstacle test and rotating rod fatigue test were used to observe the motor coordination ability and learning ability of mice.Electrophysiological patch clamp technique was used to detect the field potential changes of long-term synaptic plasticity induced by blowing stimulation.SPSS 22.0 software was used for statistical analysis.Independent sample t-test,paired t-test and repeated measurement analysis of variance were used for comparison between the two groups before and after intervention.Results The electrophysiological results showed that the amplitude percentage of field potential N1 wave in the control group after blowing stimulation was(130.4±3.3)%,which was higher than that before stimulation((100.6±2.7)%)(t=27.07,P<0.01).And the percentage of area under N1 standardized waveform after stimulation((128.8±4.5)%)was greater than that before stimulation((100.2±3.5)%)(t=19.43,P<0.01).There was no significant difference in the amplitude percentage of N1 wave in alcohol group((97.8±4.3)%)after blowing stimulation compared with that before stimulation((99.5±5.6)%)(t=0.93,P>0.05).And also there was no significant difference in the area percentage under N1 wave after stimulation((96.8±3.6)%)compared with that before stimulation((100.2±4.2)%)(t=2.38,P>0.05).The results of walking obstacle test showed that the total number of errors(3.14±0.19)in the alcohol group was higher than that in the control gr

关 键 词：酒精 小脑皮质 感觉刺激 突触可塑性 运动协调能力 小鼠 

分 类 号：R749.62[医药卫生—神经病学与精神病学]