胱抑素C对缺血再灌注损伤大鼠脑水肿和微血管的影响  

Effects of Cystatin C on brain edema and microvessels in rats with cerebral ischemia reperfusion injury

作　　者：陈萌 梁秀军[2] 王江南[3] 马凯旋 CHEN Meng;LIANG Xiujun;WANG Jiangnan(Department of Electron Microscope of Basic Medical College,Chengde 067000,China)

机构地区：[1]承德医学院基础医学院电镜室,河北承德067000 [2]承德医学院基础医学研究所,河北承德067000 [3]承德医学院,河北承德067000 [4]承德医学院河北省神经损伤与修复重点实验室,河北承德067000

出　　处：《中风与神经疾病杂志》2022年第4期292-296,共5页Journal of Apoplexy and Nervous Diseases

基　　金：河北省卫计委医学科学研究重点课题(20181145);河北省自然科学基金项目(H2015406046);承德医学院大学生创新创业训练计划项目(No.201728)。

摘　　要：目的观察胱抑素C(Cys C)干预对脑缺血再灌注损伤大鼠脑皮质AQP4、MMP-2和血脑屏障的形态学变化并探讨其影响机制。方法雄性SD大鼠随机分为假手术组、缺血再灌注组和Cys C低、中、高浓度组。采用改良线拴法制备大鼠大脑中动脉栓塞模型,缺血2 h再灌注24 h后采用干湿重法测定脑组织含水量、检测脑组织EB含量,评价血脑屏障通透性改变程度。在电镜下观察各组大鼠脑皮质微血管的形态结构;Western blot法检测水AQP4表达,免疫组化法进行MMP-2光密度值检测。结果与缺血再灌注组比较,Cys C低、中浓度组血脑屏障损伤程度明显减轻,毛细血管张开数量增多;AQP4蛋白表达降低,MMP-2表达的OD值也显著减少。而Cys C高浓度组血脑屏障损伤严重;AQP4蛋白表达明显升高,MMP-2蛋白表达OD值也明显增大。结论Cys C在一定浓度下干预能减轻缺血再灌注对血脑屏障的损伤,其机制可能与AQP4蛋白表达降低及MMP-2表达OD值减少有一定的关系。Objective To investigate the morphological changes of Cys C intervention on cerebral cortex AQP4,MMP-2 and BBB ultrastructure after cerebral ischemia reperfusion injury(CIRI)in rats and to explore its underlying mechanisms.Methods One hundred male SD rats were randomly divided into sham group,IR group,Cys C low,medium and high concentration group.The rat middle cerebral artery embolism model was established by the modified Longa occlusion method.The water content of brain tissue was measured by dry and wet weight method,the content of EB in brain tissue was detected,and the degree of permeability change of the BBB was evaluated by focal ischemia for 2 h with middle cerebral artery occlusion and 24 h reperfusion.TEM was used to observe the ultrastructure changes of BBB.The opening of microvessels was observed under SEM;Western blot was used to detect AQP4 expression in cerebral cortex tissue.MMP-2 was detected expression by immune histochemistry.Results Compared with IR group,the degree of BBB injury in Cys C low、medium groups was significantly reduced,and the number of capillaries opening increased.The expression of AQP4 protein was decreased,and the OD value of MMP-2 expression was also significantly decreased.In Cys C high group,the BBB was severely damaged.AQP4 protein expression was significantly increased,and the OD value of MMP-2 expression was also significantly increased.Conclusions The application of Cys C intervention can reduce the damage of the BBB.It is mechanism may be relation to the decrease of AQP4 protein expression and the decrease of OD value of MMP-2 expression.

关 键 词：脑缺血再灌注 AQP4蛋白 血脑屏障 MMP-2蛋白 

分 类 号：R743.3[医药卫生—神经病学与精神病学]