转录因子Kaiso下调人乳腺癌细胞系中lncRNA MEG3的表达  

Transcription factor Kaiso down-regulates the expression of lncRNA MEG3 in human breast cancer cell lines

作　　者：谢利华 杨奇 李雅倩 谢心悦 雷诗娟 周琳[1] XIE Li-hua;YANG Qi;LI Ya-qian;XIE Xin-yue;LEI Shi-juan;ZHOU Lin(Department of Clinical Biochemistry, Clinical Laboratory Medicine of Changsha Medical University, Changsha 410219,China)

机构地区：[1]长沙医学院医学检验学院临床生物化学教研室,湖南长沙410219

出　　处：《基础医学与临床》2022年第5期758-762,共5页Basic and Clinical Medicine

基　　金：湖南省自然科学基金(2020JJ5625);湖南省教育厅一般项目(18C1182);湖南省大学生创新性课题(S201910823019);湖南省卫健委科研计划课题(20190473)。

摘　　要：目的探讨转录因子Kaiso是否调节人母系表达基因3(MEG3)表达及其在乳腺癌的发生发展中发挥重要作用。方法RT-qPCR检测正常乳腺细胞系MCF-10A和多个乳腺癌细胞系MCF-7、BT-474、MDA-MB-435、MDA-MB-231中Kaiso和MEG3的表达;蛋白印迹检测Kaiso在各种细胞中的表达;免疫组织化学检测50例乳腺癌和癌旁组织中Kaiso表达;RT-qPCR检测过表达Kaiso对母系印记基因编码的长链非编码RNA(lncRNA MEG3)转录水平的影响。结果MCF-10A中lncRNA MEG3转录水平高于Kaiso的转录水平(P<0.001),在MCF-7、BT-474、MDA-MB-435、MDA-MB-231细胞中Kaiso明显上调,而lncRNA MEG3明显下调(P<0.001);MCF-10A中Kaiso表达水平较低,而在MDA-MB-231高转移性乳腺癌细胞中呈现出高表达状态(P<0.05)。Kaiso在乳腺癌旁组织中为低表达(P<0.001),而在癌组织中高表达于细胞质与细胞核中。另外在MCF-10A细胞中使Kaiso过表达后lncRNA MEG3的转录水平明显下调(P<0.01)。结论Kaiso调节lncRNA MEG3在乳腺癌组织细胞中的表达,在乳腺癌的发生发展中可能发挥重要作用。Objective To investigate the potential regualation of the expression of human maternal gene 3(MEG3)by transcription factor Kaiso and the impact on the occurrence and development of breast cancer.Methods The expression of Kaiso and MEG3 in breast cell line MCF-10a and different breast cancer cell lines MCF-7,BT-474,MDA-MB-435,MDA-MB-231 were detected by RT-qPCR.The expression of Kaiso in cells was detected by Western blot.Kaiso expression of 50 samples from cancer and paracancer tissues were examined by Western blot.RT-qPCR was used to detect the effect of Kaiso over-expressed on the transcription of long non-coding RNA(lncRNA)MEG3.Results The transcription of lncRNA MEG3 in MCF-10a was higher than that of Kaiso(P<0.001).Kaiso was significantly up-regulated in MCF-7,BT-474,MDA-MB-435,and MDA-MB-231 breast cancer cells,while lncRNA MEG3 was significantly down-regulated(P<0.001).Kaiso expression level was low in MCF-10a,but was high in MDA-MB-231 highly metastatic breast cancer(P<0.05).Kaiso was low in paracancerous breast tissues(P<0.001),but highly expressed in cytoplasm and nucleus in cancer tissues.In addition,Kaiso over-expression in MCF-10a significantly down regulated the transcription of lncRNA MEG3(P<0.01).Conclusions Kaiso regulates the expression of lncRNA MEG3 by breast cancer cells,which may play an important role in the occurrence and development of breast cancer.

关 键 词：Kaiso 长链非编码RNA(lncRNA) 母系表达基因3(MEG3) 乳腺癌 

分 类 号：R737.9[医药卫生—肿瘤]