海带多糖对放射性脑损伤小鼠海马细胞的保护作用  

Laminaria japonica polysaccharide protects hippocampal cells of mice with radiation-induced brain injury

作　　者：敖翩 张思琴 玉洪荣[2] 张馨月 谷卫丽 赵歆 王绍军[3] 韦力 Ao Pian;Zhang Siqin;Yu Hongrong;Zhang Xinyue;Gu Weili;Zhao Xin;Wang Shaojun;Wei Li(Collage of Stomatology,Guangxi Medical University,Nanning 530021;School of Basic Medicine,Guangxi Medical University,Nanning 530021;Maternity and Child Health Care of Guangxi Zhuang Autonomous Region,Nanning 530005,China)

机构地区：[1]广西医科大学口腔医学院,南宁530021 [2]广西医科大学基础医学院,南宁530021 [3]广西壮族自治区妇幼保健院,南宁530005

出　　处：《神经解剖学杂志》2022年第1期51-56,共6页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(81460479);广西自然科学基金(2018JJA140600)。

摘　　要：目的:探讨海带多糖(LJP)对放射后小鼠认知功能的影响及其影响机制。方法:64只雄性昆明小鼠利用完全随机数字法分为对照组(control)、海带多糖组(LJP)、单纯照射组(IR)、照射+海带多糖组(IR+LJP)。放射前7 d连续给药预处理后,采用总剂量30 Gy的钴60(^(60)Co)-γ射线对小鼠脑部进行定位照射以建立小鼠放射性脑损伤模型。通过Morris水迷宫检测小鼠学习记忆能力,苏木素-伊红(HE)染色观察小鼠海马齿状回(DG)组织病理学改变,电镜观察颗粒细胞超微结构变化,免疫荧光染色检测小鼠海马DG区小胶质细胞、星形胶质细胞的活化情况。结果:与对照组相比,照射后小鼠穿越平台的次数及在中心环活动时间均明显减少,而经LJP干预后上述情况得到改善;与IR组相比,经LJP干预有效改善了照射后齿状回颗粒细胞双层核膜结构的稳定,且有效改善了胶质细胞胞体形态、突起分支数量。结论:海带多糖对放射后小鼠认知功能具有保护作用,其机制可能与颗粒细胞及胶质细胞维持神经元的稳定有关。Objective:To explore the effect and mechanism of laminaria japonica polysaccharide(LJP)on cognitive function of radiated mice.Methods:Sixty-four male Kunming mice were randomly divided into control group,irradiation(IR)group,LJP group,and irradiation+LJP(IR+LJP)group.After 7 days of continuous administration and pretreatment,a total dose of 30 Gy of Cobalt-60(^(60)Co)-γrays was used to establish a radiation brain injury model in mice.The Morris water maze was performed to evaluate the learning and memory ability of mice;HE staining was performed to observe pathological changes in the dentate gyrus of the hippocampus;electron microscopy was used to examine ultrastructure changes in granular cells;immunofluorescence staining was used to observe the activation of microglia and astrocyte in the dentate gyrus of the hippocampus.Results:Compared with the control group,the times of acrossing the platform and the time of staying in the middle ring reduced significantly in IR and IR+LJP groups,while LJP intervention improved the above-mentioned situation;compared with the IR group,the LJP intervention alleviated the stability of double nuclear membrane structure of granule cells,and improved the morphology and the processes’length of glial cells after irradiation.Conclusion:Radioprotective effects of LJP on cognition impairment in mice may be related with granular cells and glial cells that could maintain the stability of neurons.

关 键 词：海带多糖 认知障碍 齿状回 颗粒细胞 胶质细胞 神经元 小鼠 

分 类 号：R285.5[医药卫生—中药学]