TLR4-NLRP3介导蛛网膜下腔出血早期大鼠神经细胞焦亡  

TLR4-NLRP3 mediates pyroptosis of rat nerve cells in the early stage of subarachnoid hemorrhage

作　　者：郝广志 邹正 陈立刚 韩雨薇 董玉书 Hao Guangzhi;Zou Zheng;Chen Ligang;Han Yuwei;Dong Yushu(Institute of Neuroscience,General Hospital of Northern Theater Command Base,Shenyang 110016,China)

机构地区：[1]中国人民解放军北部战区总医院神经外科,沈阳110016

出　　处：《神经解剖学杂志》2022年第1期74-78,共5页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(82071481);全军医学科技青年培育计划拔尖项目(17QNP053)。

摘　　要：目的:研究蛛网膜下腔出血大鼠(SAH)早期神经细胞焦亡的分子机制。方法:雄性SD大鼠分为假手术组和SAH组。利用注射自体血液的方法制备SAH大鼠模型,利用Western Blot检测大鼠皮层toll样受体4(TLR4)、NOD样受体蛋白3(NLRP3)和活化型gasdermin D(GSDMD-p30)的表达,利用商品化试剂盒检测caspase-1的活性,利用酶联免疫吸附试验(ELISA)检测大鼠脑脊液中白细胞介素-1β(IL-1β)和白细胞介素-18(IL-18)的含量。结果:SAH术后48 h大鼠皮层中TLR4、NLRP3的表达增加明显(P<0.05),caspase-1的活性升高(P<0.05),同时GSDMD0-p30升高,脑脊液中IL-1β和IL-18含量明显增加(P<0.05)。结论:SAH早期大鼠皮层发生了TLR4-NLRP3介导的细胞焦亡。Objective:To study the molecular mechanism of pyroptosis of rat nerve cells in the early stage of subarachnoid hemorrhage(SAH).Methods:Male SD rats were divided into sham operation group and SAH group.The expression of toll like receptor 4(TLR4),Nod-liker receptor protein 3(NLRP3),and cleaved gasdermin D(GSDMD-p30)in the rat cortex was detected by Western Blot.The activity of caspase-1 was detected by commercial kit.The contents of interleukin-1β(IL-1β)and interleukin-18(IL-18)in cerebrospinal fluid(CSF)were determined by enzyme-linked immunosorbent assay(ELISA).Results:The expression of TLR4,NLRP3,and GSDMD-p30 increased significantly in the cortex 48 h after SAH(P<0.05).The activity of caspase-1 increased significantly(P<0.05),and the content of IL-1βand IL-18 in CSF increased significantly(P<0.05).Conclusion:TLR4-NLRP3-mediated pyroptosis occurs in the cortex of early SAH rats.

关 键 词：焦亡 TOLL样受体4 NLRP3 蛛网膜下腔出血 早期脑损伤 大鼠 

分 类 号：R743.35[医药卫生—神经病学与精神病学]