22RV1型前列腺癌荷瘤裸鼠模型的制作及^(18)F-FPA PET/CT的显像研究  

Establishment of 22RV1 tumor bearing nude mouse model of prostate cancer and ^(18)F-FPA PET/CT imaging study

作　　者：王朋[1,2] 齐红艳 谭支娥[1] 邓鹏裔 冯亚琪 陈海波 代文莉 WANG Peng;QI Hongyan;TAN Zhi'e;DENG Pengyi;FENG Yaqi;CHEN Haibo;DAI Wenli(The First College of Clinical Medical Science,China Three Gorges University,Yichang Central People's Hospital,Key Laboratory of Nuclear Medicine and Molecular Imaging of Yichang,Hubei Yichang 443003,China;Zhijiang People's Hospital,Hubei Yichang 443200,China)

机构地区：[1]三峡大学第一临床医学院,宜昌市中心人民医院,宜昌市核医学分子影像重点实验室,湖北宜昌443003 [2]枝江市人民医院,湖北宜昌443200

出　　处：《现代肿瘤医学》2022年第10期1717-1722,共6页Journal of Modern Oncology

基　　金：湖北省自然科学基金项目(编号:2018CFB147);湖北省宜昌市医疗卫生研究项目(编号:A20-2-005,A13301-12)。

摘　　要：目的:探讨22RV1型前列腺癌荷瘤裸鼠模型的制作,以及^(18)F-FPA在裸鼠模型中的显像价值。方法:选取22RV1型前列腺癌细胞株进行培养,饲养BALB/c型裸鼠10只,将指数生长期细胞接种于裸鼠右前臂皮下,定期观察成瘤情况。利用CFN-200多功能模块合成显像剂^(18)F-FPA,然后对荷瘤裸鼠进行PET/CT显像,并与^(18)F-FDG和^(11)C-胆碱显像进行对比,评价^(18)F-FPA对前列腺癌的显像价值。结果:BALB/c型裸鼠一次接种的成瘤率为80%(8/10),经一次补种后成瘤率达100%(10/10)。^(18)F-FPA的合成过程约40 min,放射化学纯度大于97%,符合显像要求。静脉注射显像剂^(18)F-FPA后0.5 h、1 h、2 h,肿瘤均能够清晰显像,最大标准化摄取值(SUV_(max))分别为5.79±0.78、6.22±0.95、6.29±0.94,无明显统计学差异。对比显像显示,肿瘤对^(18)F-FDG和^(11)C-胆碱仅有轻微摄取,SUV_(max)均小于^(18)F-FPA显像(P<0.01)。结论:用22RV1型前列腺癌细胞株和BALB/c裸鼠可成功制作前列腺癌荷瘤裸鼠模型。^(18)F-FPA在22RV1型前列腺癌荷瘤裸鼠模型上有良好的显像效果,但还需要和其他新型显像剂做进一步对比研究。Objective:To investigate the establishment of 22RV1 tumor bearing nude mouse model of prostate cancer and the imaging value of ^(18)F-FPA.Methods:22RV1 prostate cancer cell lines were selected for culture,and 10 BALB/c nude mice were raised.The exponential growth period cells were inoculated under the skin of the right forelimb of nude mice,and tumor formation was regularly observed.The imaging agent ^(18)F-FPA was synthesized by CFN-200 multifunctional module,and then the imaging of tumor-bearing nude mice was performed,and compared with ^(18)F-FDG and ^(11)C-choline imaging,to evaluate the imaging value of ^(18)F-FPA for prostate cancer.Results:The tumorigenesis rate was 80%(8/10)after the first inoculation and 100%(10/10)after the reseeding.The synthesis process of ^(18)F-FPA was about 40 min,and the radiochemical purity was over 97%,which met the imaging requirements.Tumor imaging could be clearly developed at 0.5 h,1 h and 2 h after intravenous injection of imaging agent ^(18)F-FPA,and the maximum standardized uptake values(SUV_(max))were 5.79±0.78,6.22±0.95 and 6.29±0.94 respectively,showing no significant statistical difference(P>0.05).Contrast imaging showed that the tumor had only slight uptake of ^(18)F-FDG and ^(11)C-choline,and SUV_(max) was lower than that of ^(18)F-FPA(P<0.01).Conclusion:22RV1 prostate cancer cell line and BALB/c nude mice can be successfully used to develop the tumor-bearing model of prostate cancer in nude mice.^(18)F-FPA has a good imaging effect in 22RV1 type prostate cancer nude mouse model,but it needs to be further compared with other novel imaging agents.

关 键 词：前列腺癌 裸鼠 ^(18)F-FPA PET/CT 

分 类 号：R737.25[医药卫生—肿瘤]