基于网络药理学的参芪扶正注射液治疗癌因性疲乏作用机制探析  

Shenqi Fuzheng Injection therapeutic mechanism for cancer-related fatigue based on network pharmacology

作　　者：张涛 苏丽群 卢建辉 聂多锐 石斌 陈楚瑶 黄越 蓝清霞 黄学武[3] 肖志伟[3] ZHANG Tao;SU Liqun;LU Jianhui;NIE Duorui;SHI Bin;CHEN Chuyao;HUANG Yue;LAN Qingxia;HUANG Xuewu;XIAO Zhiwei(The First Clinical Medicine College of Guangzhou University of Chinese Medicine,Guangdong Guangzhou 510405,China;Graduate School of Guangxi University of Chinese Medicine,Guangxi Nanning 530001,China;the First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangdong Guangzhou 510405,China)

机构地区：[1]广州中医药大学第一临床医学院,广东广州510405 [2]广西中医药大学研究生院,广西南宁530001 [3]广州中医药大学第一附属医院,广东广州510405

出　　处：《现代肿瘤医学》2022年第10期1840-1846,共7页Journal of Modern Oncology

基　　金：广东省中医药管理局项目(编号:20172044);广东省名中医传承工作室建设项目(编号:2018[5]号)。

摘　　要：目的:探讨参芪扶正注射液(Shenqi Fuzheng Injection,SFI)治疗癌因性疲乏(cancer-related fatigue,CRF)的物质基础与潜在的作用机制。方法:利用TCMSP数据库筛选SFI的活性成分及对应作用靶点基因,检索GeneCards数据库获得疾病CRF相关靶点基因,将两者映射得到SFI作用于CRF的预测靶基因。利用Cytoscape 3.7.2构建SFI活性成分-作用靶点-疾病网络图,采用STRING数据库构建SFI作用于CRF的预测靶基因蛋白互作网络并进行模块分析和关键基因筛选。利用Clue GO插件进行GO分析和KEGG通路富集分析。结果:获得参芪扶正注射液治疗癌因性疲乏的共27个成分,包括槲皮素(quercetin)、山奈酚(kaempferol)等,主要作用于TP53、IL-6、JUN、VEGFA、MAPK1、PTGS26个关键靶基因,涉及AGE-RAGE、IL-17、TNF、Relaxin、CLR、HIF-1、TCR等信号通路,通过改善氧化应激和线粒体功能障碍、调控免疫激活及炎症反应、调节细胞因子等过程共同发挥抗CRF作用。结论:本研究通过网络药理学的方法初步预测了参芪扶正注射液治疗癌因性疲乏的分子机制,可为后续临床及基础研究提供参考。Objective:To explore the material basis and potential mechanism of Shenqi Fuzheng Injection(SFI)for cancer-related fatigue(CRF)treatment.Methods:TCMSP database was used to obtain the active components and target genes of SFI,while GeneCards database was retrieved for the target genes of CRF,and we mapped the two groups to obtain the common target genes of disease-drugs groups.Cytoscape 3.7.2 software was used to construct the interrelationship network of compound-target-disease.The protein interaction network of common target genes was analyzed by using STRING database,and Module analysis and key gene screening were conducted.Gene ontology and KEGG enrichment analysis was performed by using Clue GO.Results:Twenty-seven active ingredients,including quercetin,kaempferol,were screened from SFI.They mainly act on six targets:TP53,IL-6,JUN,VEGFA,MAPK1 and PTGS2,which play a role in the treatment of CRF by interfering with oxidative stress and mitochondrial dysfunction,immunity,inflammation and cytokinesthrough different signal pathways such as AGE-RAGE,IL-17,TNF,Relaxin,CLR,HIF-1 and TCR.Conclusion:This study preliminarily predicted the active components,key targets and the molecular mechanism of SFI in the treatment of CRF through network pharmacology,which can provide reference for subsequent clinical and basic research.

关 键 词：参芪扶正注射液 癌因性疲乏 网络药理学 作用机制 

分 类 号：R730.53[医药卫生—肿瘤]