卵巢癌耐药相关的miRNAs筛选及其预后意义研究  

Identification of miRNAs Associated with Chemotherapy Resistance in Ovarian Cancer and Its Prognostic Significance

作　　者：朱轶昊 杨奕辰 马琳琳 ZHU Yi-hao;YANG Yi-chen;MA Lin-lin(School of Medical Technology,Shanghai University of Medicine&Health Sciences,Shanghai 201318,China;School of Information Science and Technology,Shanda University,Shanghai 201209,China)

机构地区：[1]上海健康医学院医学技术学院,中国上海201318 [2]上海杉达学院信息科学与技术学院,中国上海201209

出　　处：《生命科学研究》2022年第2期158-164,共7页Life Science Research

摘　　要：卵巢癌常因耐药复发,严重影响患者预后。本研究首先利用TCGA数据库下载卵巢癌的转录组数据及对应的临床信息,通过差异分析筛选出与卵巢癌耐药相关的miRNAs;其次,利用单因素Cox比例风险回归分析及Kaplan-Meier分析探讨这些miRNAs的预后价值,并通过多因素Cox比例风险回归分析构建预后风险评分模型;最后,基于预后风险评分及临床病理参数构建预测卵巢癌死亡风险的列线图模型。差异分析共筛选出84个与卵巢癌耐药相关的miRNAs。单因素Cox比例风险回归分析、Kaplan-Meier分析和前向逐步回归法筛选出8个与卵巢癌预后相关的miRNAs。构建的预后风险评分模型在预测复发风险上具有较好的敏感性及特异性,预后风险评分是影响卵巢癌患者预后的独立风险因子。基于风险评分及临床病理参数构建的列线图模型能够精准地对卵巢癌患者的死亡风险进行预测。Ovarian cancer often relapses due to chemotherapy resistance,seriously affecting the prognosis of patients.To identify miRNAs related to chemotherapy resistance of ovarian cancer and explore their prognostic value,the differentially expressed miRNAs related to chemotherapy resistance of ovarian cancer were screened from TCGA database.Univariate Cox proportional risk regression analysis and Kaplan-Meier analysis were used to screen out miRNAs related to prognosis from those screened miRNAs.A prognostic risk score model was constructed by multivariate Cox proportional risk regression analysis.Finally,a nomogram model was constructed based on the prognostic risk score and clinicopathologic parameters.Eighty-four miRNAs related to chemotherapy resistance of ovarian cancer were screened through differential analysis,and 8 miRNAs related to the prognosis of ovarian cancer were screened out by univariate Cox proportional risk regression analysis,Kaplan-Meier analysis and forward stepwise regression.The results demonstrated that the constructed prognostic risk score model had good sensitivity and specificity in predicting the recurrence risk,and that the risk score was an independent prognostic factor.The nomogram model based on the risk score and clinicopathologic parameters would be helpful in predicting the mortality risk of ovarian cancer accurately.

关 键 词：卵巢癌 化疗耐药性 微RNA(miRNA) 预后风险评分模型 列线图模型 

分 类 号：Q811.4[生物学—生物工程] R737.31[医药卫生—肿瘤]