微波消解-电感耦合等离子体质谱法测定胎盘组织17种元素  

Determination of 17 elements in placenta by microwave digestion-inductively coupled plasma mass spectrometry

作　　者：徐文珍[1] 罗亚翠 张海君[2] 方家阳 张海蕾[2] 张双凤[3] XU Wenzhen;LUO Yacui;ZHANG Haijun;FANG Jiayang;ZHANG Hailei;ZHANG Shuangfeng(Department of Quality Management,Taizhou Center for Disease Control and Prevention,Taizhou,Zhejiang 318000,China;Taizhou Center for Disease Control and Prevention,Taizhou,Zhejiang 318000,China;Zhejiang Provincial Center for Disease Control and Prevention,Hangzhou,Zhejiang 310051,China)

机构地区：[1]台州市疾病预防控制中心质管科,浙江台州318000 [2]台州市疾病预防控制中心,浙江台州318000 [3]浙江省疾病预防控制中心,浙江杭州310051

出　　处：《预防医学》2022年第5期534-539,共6页CHINA PREVENTIVE MEDICINE JOURNAL

基　　金：台州市科技局项目(1902ky91)。

摘　　要：目的建立微波消解-电感耦合等离子体质谱法同时测定人胎盘中多种元素。方法胎盘组织60℃干燥15 h后均质磨粉。称取0.3 g均质后样品,采用微波消解技术消解。采用电感耦合等离子体质谱的动能甄别模式消除质谱干扰,在线内标模式消除基质干扰后,检测胎盘组织中钒(V)、镍(Ni)、钴(Co)、铁(Fe)、铬(Cr)、铜(Cu)、锌(Zn)、锰(Mn)、砷(As)、锡(Sn)、锑(Sb)、钡(Ba)、硒(Se)、镉(Cd)、铅(Pb)、汞(Hg)和铊(Tl)17种元素。结果V、Ni、Co、Cr、As、Se、Cd、Pb、Tl在1~50μg/L,Fe在100~5000μg/L,Zn、Cu、Mn、Ba在10~500μg/L,Sn、Sb在0.1~5μg/L,Hg在0.2~2μg/L范围内线性关系良好,相关系数均≥0.9998。17种元素的检出限为0.5~100μg/kg,相对标准偏差(RSD)为2.1%~6.5%,回收率为83.3%~110.0%。以鸡肉标准物质(GBW10018)辅证方法准确性和适用范围,各元素测定结果均在证书标准值范围内。结论通过优化前处理方法和质谱检测条件建立的微波消解-电感耦合等离子体质谱法适用于胎盘中多种元素的检测。Objective To establish a microwave digestion-inductively coupled plasma mass spectrometry assay for simul⁃taneous determination of multiple elements in placenta.Methods Fresh placental tissues were dried at 60℃for 15 h and ground into power.Then,0.3 g homogenized samples were digested in a microwave digestion system.The interfer⁃ence from mass spectrometry was removed using the kinetic energy discrimination model in the inductively coupled plas⁃ma mass spectrometry,and the baseline interference was removed by online internal standards.The contents of 17 ele⁃ments were determined in placental specimens using the established microwave digestion-inductively coupled plasma mass spectrometry assay,including V,Ni,Co,Fe,Cr,Cu,Zn,Mn,As,Sn,Sb,Ba,Se,Cd,Pb,Hg and Tl.Results Good linearity was shown for V,Ni,Co,Cr,As,Se,Cd,Pb and Tl at 1 to 50μg/L,Fe at 100 to 5000μg/L,Zn,Cu,Mn and Ba at 10 to 500μg/L,Sn and Sb at 0.1 to 5μg/L,and Hg at 0.2 to 2μg/L,with all correlation coeffi⁃cients of 0.9998 and higher.The detection limits of these 17 elements ranged from 0.5 to 100μg/kg,with relative standard deviations of 2.1%to 6.5%,and recovery rates of 83.3%to 110.0%.The determination results of 17 elements were all within the normal reference range defined in the certified reference materials of chicken(GBW10018).Conclu⁃sions The microwave digestion-inductively coupled plasma mass spectrometry established based on optimized pretreat⁃ments and mass spectrometry detection conditions,is feasible for simultaneous determination of multiple elements in placenta.

关 键 词：胎盘 元素 微波消解 电感耦合等离子体质谱法 

分 类 号：R446.1[医药卫生—诊断学]