靶向miR-188-3p/HMGB2抑制circ_0008035对肺癌细胞生物行为的影响  

Effects of inhibiting circ_0008035 on the biological behavior of lung cancer cells by targeting miR-188-3p/HMGB2

作　　者：李宁[1] 陈瑾[1] 张录民[2] LI Ning;CHEN Jin;ZHANG Lumin(Department of Respiratory Fuxing Hospital Affiliated to Capital Medical University,Beijing 100040,China;不详)

机构地区：[1]首都医科大学附属复兴医院呼吸科,北京市100040 [2]吉林省人民医院胸外科

出　　处：《河北医药》2022年第9期1297-1301,共5页Hebei Medical Journal

摘　　要：目的探讨环状RNA_0008035(circ_0008035)对肺癌细胞生物行为的影响及分子机制。方法应用实时定量聚合酶链反应(qRT-PCR)测定25例肺癌组织中circ_0008035和miR-188-3p表达。在A549细胞中转染si-NC(si-NC组)、si-circ_0008035(si-circ_0008035组)、miR-NC(miR-NC组)、miR-188-3p mimics(miR-188-3p组),或共转染si-circ_0008035和anti-miR-188-3p(si-circ_0008035+anti-miR-188-3p组)。另设正常培养的A549细胞为对照(con组)。采用Western blot测定高迁移率族蛋白B2(HMGB2)表达,细胞计数试剂盒8(CCK-8)法测定细胞增殖,平板克隆形成试验测定细胞克隆形成,流式细胞术测定细胞凋亡率,双荧光素酶活性检测分析circ_0008035与miR-188-3p、miR-188-3p与HMGB2之间的靶向关系。结果肺癌组织中circ_0008035表达较癌旁组织增加,miR-188-3p表达较癌旁组织减少(P<0.05)。与con组或si-NC组相比,si-circ_0008035组circ_0008035表达、HMGB2蛋白表达、克隆形成数减少,miR-188-3p表达、细胞抑制率与凋亡率增加(P<0.05)。与con组和miR-NC组相比,miR-188-3p组miR-188-3p表达、细胞抑制率与凋亡率增加,HMGB2蛋白表达、克隆形成数减少(P<0.05)。circ_0008035靶向miR-188-3p,miR-188-3p靶向HMGB2。si-circ_0008035+anti-miR-188-3p组miR-188-3p表达、细胞抑制率与凋亡率比si-circ_0008035组低,HMGB2蛋白表达、克隆形成数比si-circ_0008035组高(P<0.05)。结论抑制circ_0008035通过靶向miR-188-3p/HMGB2,抑制肺癌细胞增殖,和诱导细胞凋亡。Objective To investigate the effects of circular RNA_0008035(circ_0008035)on the biological behavior of lung cancer cells,and to explore its molecular mechanism.Methods Real-time quantitative polymerase chain reaction(qRT-PCR)was used to determine the expression of circ_0008035 and miR-188-3p in 25 lung cancer tissues.A549 cells were transfected with si-NC(si-NC group),si-circ_0008035(si-circ_0008035 group),miR-NC(miR-NC group),miR-188-3p mimics(miR-188-3p group),or co-transfected with si-circ_0008035 and anti-miR-188-3p(si-circ_0008035+anti-miR-188-3p group).In addition,normally cultured A549 cells were taken as control group.Western Blot was used to determine the expression of high mobility group protein B2(HMGB2),and cell counting kit 8(CCK-8)method was used to determine cell proliferation.Plate clone formation test was used to determine cell clone formation,and flow cytometry was used to determine cell apoptosis rate.Luciferase activity assay was used to detect and analyze the targeting relationship between circ_0008035 and miR-188-3p,and between miR-188-3p and HMGB2.Results The expression levels of circ_0008035 in lung cancer tissue were significantly higher than those in adjacent tissues,while the expression levels of miR-188-3p were significantly lower than those in adjacent tissues(P<0.05).Compared with those in control group or si-NC group,the circ_0008035 expression,HMGB2 protein expression,and the number of clone formation in si-circ_0008035 group were decreased,while the expression levels of miR-188-3p,the cell inhibition rate and the apoptosis rate were significantly increased(P<0.05).Compared with those in control group and miR-NC group,the miR-188-3p expression,cell inhibition rate and apoptosis rate of the miR-188-3p group were increased,while the expression of HMGB2 protein and the number of clone formation were significantly decreased(P<0.05).Due to circ_0008035 targeting miR-188-3p,and miR-188-3p targeting HMGB2,miR-188-3p expression,cell inhibition rate and apoptosis rate in the si-circ_000803

关 键 词：肺癌 增殖 凋亡 circ_0008035 miR-188-3p 高迁移率族蛋白B2 

分 类 号：R734.2[医药卫生—肿瘤]