利用大孔树脂分离灵芝菌丝体中水溶性抗氧化成分  

Macroporous Resin for Separating Water-soluble Antioxidant Components from Ganoderma lucidum Mycelium

作　　者：韩伟 郑丹婷 姚泽远 卜原玲 冯杰 HAN Wei;ZHENG Danting;YAO Zeyuan;BU Yuanling;FENG Jie(Engineering Research Center of Pharmaceutical Process Chemistry,Ministry of Education,School of Pharmacy,East China University of Science and Technology,Shanghai 200237,China;Shanghai Key Laboratory of New Drug Design,Ministry of Education,School of Pharmacy,East China University of Science and Technology,Shanghai 200237,China;National Engineering Research Centre of Edible Fungi,Institute of Edible Fungi,Shanghai Academy of Agriculture Sciences,Shanghai 201403,China)

机构地区：[1]华东理工大学药学院制药工程与过程化学教育部工程研究中心,上海200237 [2]华东理工大学药学院上海市新药设计重点实验室,上海200237 [3]华东理工大学药学院上海市农业科学院食用菌研究所国家食用菌工程技术研究中心,上海201403

出　　处：《徐州工程学院学报（自然科学版）》2022年第1期22-30,共9页Journal of Xuzhou Institute of Technology(Natural Sciences Edition)

基　　金：上海市科技兴农重点攻关项目(沪农科创字(2018)第1-1号)。

摘　　要：通过静态吸附-解吸实验对8种不同极性大孔树脂对灵芝菌丝体中水溶性抗氧化成分的吸附进行筛选,确定中等极性的HPD-400大孔树脂为最佳.对上样后的大孔树脂利用不同体积比的乙醇(30%、50%、70%、95%)进行梯度洗脱,对比不同极性洗脱液的抗氧化活性,发现30%的乙醇可以洗脱绝大部分的抗氧化活性物质.将HPD-400大孔树脂分离后得到的30%乙醇洗脱物与水提物的体外抗氧化活性EC_(50)进行对比,结果表明经大孔树脂的富集,提取液的DPPH·清除能力可提高1倍,ABTS^(+)·清除能力可提高3倍,但羟自由基的清除能力有所降低,说明大孔树脂可有效富集具有DPPH·和ABTS^(+)·清除能力的水溶性抗氧化成分.The adsorption of 8 different polar macroporous resins to the water-soluble antioxidant components in Ganoderma lucidum mycelium was screened by static adsorption-desorption experiments,and the medium-polarity HPD-400 macroporous resin was determined to be the best.Gradient elution was performed on the loaded macroporous resin with different volume ratios of ethanol(30%,50%,70%,95%).The antioxidant activity of eluates with different polarities was compared,and it was found that 30%ethanol can elute most of the antioxidant active substances.The antioxidant activity EC_(50) of 30%ethanol eluate separated by HPD-400 macroporous resin was compared with that of water extract in vitro.The result showed that after the enrichment of the microporous resin,the treatment can double the DPPH·scavenging ability of the extract.The scavenging ability of ABTS^(+)·was increased by 3 times,but the scavenging ability of hydroxyl radicals was reduced.It shows that the macroporous resin can effectively enrich and separate water-soluble antioxidant components with DPPH·and ABTS^(+)·scavenging capabilities.

关 键 词：灵芝菌丝体 体外抗氧化 大孔树脂分离 

分 类 号：TQ426[化学工程]