基于网络药理学研究宽筋藤治疗特异性皮炎的作用机制  

Study on the underlying mechanism of Kuanjinteng treating atopic dermatitis based on network pharmacology

作　　者：龚娟[1] 杨华[1] 唐雪勇 戚东卫[1] GONG Juan;YANG Hua;TANG Xue-yong;QI Dong-wei(Chongqing Hospital of Traditional Chinese Medicine,Department of Dermatology,Chongqing 400011,China)

机构地区：[1]重庆市中医院皮肤科,重庆400011

出　　处：《中药与临床》2022年第1期32-35,43,共5页Pharmacy and Clinics of Chinese Materia Medica

基　　金：重庆市科卫联合中医药科研项目(ZY201802142);国家自然科学基金项目(81704091)。

摘　　要：目的:运用网络药理学研究宽筋藤多成分、多靶点、多通络治疗特异性皮炎(Atopic dermatitis,AD)的机制。方法:使用TCMSP、BATMAN-TCM数据库以及文献调研获取宽筋藤的活性小分子以及对应的靶点,通过GeneCards,OMIM数据库分析AD疾病靶点;使用STRING以及Cytoscape 3.7.0软件获得“宽筋藤-活性小分子-靶点-AD”网络、韦恩图、PPI网络;运用R语言对筛选出的核心基因进行GO功能富集分析以及KEGG通路分析。结果:宽筋藤中共有12个活性小分子符合条件,对应228个靶点,AD相关基因2301个,113个交集靶点,GO功能富集分析主要集中在细胞因子活性及受体、蛋白激酶的调控。KEGG通路分析显示宽筋藤主要通过AGE--RAGE signal pathway,IL-17 signal pathway,Toll-like receptor signaling pathway,Th17 cell dierentiation发挥其治疗作用。Objective:The present study was to investigate the underlying mechanism of Kuanjinteng with multiple components,multiple targets,and multiple pathways in treating atopic dermatitis(AD)disorder by network pharmacology.Method:The active compounds of Kuanjinteng and related targets were obtained from TCMSP,BATMAN-TCM databases and literature research.GeneCards and OMIM database were used to screen AD-related genes.“Kuanjinteng-compounds-targets-AD”network,venn diagram,and PPI network were constructed through STRING and Cytoscape 3.7.0 software.R language was used to perform GO function enrichment analysis and KEGG pathway analysis.Result:228 genes corresponding to 12 active compounds and 2301 AD related-genes were screened.There were 113 intersection genes.GO function enrichment analysis showed that cytokine activity,cytokine receptors and protein kinases were mainly involved in the therapeutic effects of Kuanjinteng.AGERAGE signal pathway,IL-17 signal pathway,Toll-like receptor signaling pathway and Th17 cell differentiation might be the main signal pathways mediated by Kuanjinteng.

关 键 词：宽筋藤 AD 网络药理学 作用机制 

分 类 号：R285.5[医药卫生—中药学]