H3K27M及其甲基化在弥漫性脊髓胶质瘤中的表达及与预后的关系  

Relationship between prognosis and the expression of H3K27M and its methylation in diffuse spinal glioma

作　　者：宫睿[1] 刘暌 陈劲草[1] 江普查[1] GONG Rui;LIU Kui;CHEN Jingcao;JIANG Pucha(Department of Neurosurgery,Zhongnan Hospital of Wuhan University,Wuhan 430071,China)

机构地区：[1]武汉大学中南医院神经脊柱外科,武汉430071

出　　处：《中国癌症防治杂志》2022年第2期167-170,共4页CHINESE JOURNAL OF ONCOLOGY PREVENTION AND TREATMENT

基　　金：湖北省自然科学基金面上项目(2018CFB646)。

摘　　要：目的探讨可催化组蛋白H3第27位(histone H3 lysine 27-to-methionine mutations,H3K27M)及其甲基化在弥漫性脊髓胶质瘤中的表达及其与预后的关系。方法选择武汉大学中南医院2016年1月—2019年1月收治的75例弥漫性脊髓胶质瘤患者为研究对象,采用免疫组化和染色质免疫沉淀联合芯片法检测胶质瘤组织中H3K27M、H3K27M三甲基化(H3K27me3)表达情况,并利用多因素Cox回归分析H3K27M、H3K27me3表达水平与总生存期的关联。结果弥漫性脊髓胶质瘤组织中H3K27M阳性表达率为65.33%,H3K27me3高表达率为58.67%。H3K27M及H3K27me3表达水平均与WHO分级、颅内播散转移有关(均P<0.05)。H3K27M阳性组2年生存率低于H3K27M阴性组(51.6%vs 78.9%,χ^(2)=7.954,P=0.005),H3K27me3高表达组2年生存率低于H3K27me3低表达组(48.4%vs 74.2%,χ^(2)=8.130,P=0.004)。多因素Cox回归校正潜在的协变量后,H3K27M阳性表达组的死亡风险较H3K27M阴性表达组高67%(HR=1.67,95%CI:1.06~2.64,P=0.026),H3K27me3高表达组的死亡风险较H3K27me3低表达组高84%(HR=1.84,95%CI:1.05~3.22,P=0.032)。结论弥漫性脊髓胶质瘤组织中H3K27M呈阳性表达,H3K27me3呈高表达,两者均与较差的预后相关。ObjectiveTo investigate the expression of histone H3 lysine 27-to-methionine mutations (H3K27M) and its methylation in diffuse spinal glioma and its relationship with prognosis.MethodsA total of 75 patients with diffuse spinal glioma admitted to Zhongnan Hospital of Wuhan University from January 2016 to January 2019 were selected as the research subjects.The expression of H3K27M and H3K27M trimethylation (H3K27me3) in glioma tissues was detected by immunohistochemistry and chromatin immunoprecipitation combined with microarray assay.The multivariable Cox regression analysis was performed to study the association between the expression levels of H3K27M and H3K27me3 and the overall survival.ResultsThe positive expression rate of H3K27M was 65.33% and the high expression rate of H3K27me3 was 58.67% in diffuse spinal glioma tissues.The expression levels of H3K27M and H3K27me3were related to WHO grade and intracranial spread and metastasis (all P<0.05).The 2-year survival rate of the H3K27M positive group was lower than that of the H3K27M negative group (51.6% vs 78.9%,χ^(2)=7.954,P=0.005),and the 2 - year survival rate of the H3K27me3 high expression group was lower than that of the H3K27me3 low expression group (48.4% vs 74.2%,χ^(2)=8.130,P=0.004).After adjusted for potential covariates by multivariable Cox regression,the H3K27M positive expression group had a risk of death 67%higher than that of the H3K27M negative expression group (HR=1.67,95%CI:1.06-2.64,P=0.026),and the H3K27me3 high expression group had a risk of death 84% higher than that of the H3K27me3 low expression group (HR=1.84,95%CI:1.05-3.22,P=0.032).ConclusionsThe H3K27M is positively expressed and H3K27me3 is highly expressed in diffuse spinal glioma tissues,both of which are associated with poor prognosis.

关 键 词：弥漫性脊髓胶质瘤 可催化组蛋白H3第27位 甲基化 

分 类 号：R739.41[医药卫生—肿瘤]