非肌层浸润性膀胱癌微小RNA-138、微小RNA-143表达与患者预后的相关性分析  

Correlations of microRNA-138 and microRNA-143 expression with prognosis in patients with non-muscle invasive bladder cancer

作　　者：张刚[1] 任衢军 成泽民[1] ZHANG Gang;REN Qujun;CHENG Zemin(Department of Urological Surgery,Dazhou Central Hospital of Sichuan Province,Dazhou,Sichuan,635000)

机构地区：[1]四川省达州市中心医院泌尿外科,四川达州635000

出　　处：《实用临床医药杂志》2022年第8期86-90,94,共6页Journal of Clinical Medicine in Practice

基　　金：四川省达州市市级医学科研课题面上项目(201102)。

摘　　要：目的探讨非肌层浸润性膀胱癌(NMIBC)组织中微小RNA-138(miR-138)、微小RNA-143(miR-143)表达情况与预后的相关性。方法选取124例NMIBC患者作为研究对象,其中68例患者同时取癌组织与癌旁组织(距离病灶约3 cm)。采用实时逆转录聚合酶链反应(RT-PCR)法检测NMIBC组织、癌旁组织中miR-138、miR-143表达水平。术后随访36个月,分析NMIBC患者的预后情况,应用多元Cox回归模型分析预后的危险因素。采用Log-rankχ^(2)检验比较miR-138、miR-143不同表达量患者的生存率,并采用Kappa检验分析NMIBC组织中miR-138与miR-143表达的一致性。结果NMIBC组织中miR-138、miR-143表达量低于癌旁组织,差异有统计学意义(P<0.05)。多元Cox回归分析显示,肿瘤T_(1)期、肿瘤直径≥3 cm、多发肿瘤、高级别乳头状尿路上皮癌(UPC)是预后的危险因素(P<0.05),而miR-138高表达、miR-143高表达是预后的保护因素(P<0.05)。miR-138、miR-143高表达患者的无瘤生存率与累积生存率均高于miR-138、miR-143低表达患者,差异有统计学意义(P<0.01)。Kappa检验结果提示,NMIBC组织中miR-138表达与miR-143表达具有高度一致性(P<0.01)。结论NMIBC组织中miR-138、miR-143表达均下调,而miR-138、miR-143表达降低可增加NMIBC患者预后不良风险,降低无瘤生存率与累积生存率。Objective To investigate correlations of expressions of microRNA-138(miR-138)and microRNA-143(miR-143)with prognosis in patients with non-muscle invasive bladder cancer(NMIBC).Methods A total of 124 patients with NMIBC were included as research objects,and tumor tissue and adjacent tissue(about 3 cm away from the lesion)were taken from 68 patients simultaneously.The expression levels of miR-138 and miR-143 in cancer tissues and adjacent tissues were detected by real-time reverse transcription polymerase chain reaction(RT-PCR).NMIBC patients were followed up for 36 months after surgery.Multivariate Cox regression model was used to analyze the prognostic risk factors.Log-rank chi-square test was used to analyze the correlation of miR-138 and miR-143 expression with prognosis.Kappa test was used to analyze the consistency of miR-138 and miR-143 expression in NMIBC tissues.Results The expressions of miR-138 and miR-143 in NMIBC were lower than those in adjacent tissues(P<0.05).Cox multiple regression analysis showed that tumor in T_(1) stage,tumor diameter≥3 cm,multiple tumors and high-grade papillary urothelial carcinoma(UPC)were prognostic risk factors,while the high expressions of miR-138 and miR-143 were prognostic protective factors(P<0.05).The tumor free and cumulative survival rates in patients with high expression of miR-138 and miR-143 were higher than those with low expressions of miR-138 and miR-143(P<0.01).Kappa test showed that the expression of miR-138 in NMIBC was highly consistent with that of miR-143(P<0.01).Conclusion The expressions of miR-138 and miR-143 in NMIBC are down regulated,while decreased expressions of miR-138 and miR-143 could increase the risk of poor prognosis and reduce the tumor-free and cumulative survival rate of NMIBC.

关 键 词：非肌层浸润性膀胱癌 预后 微小RNA-138 微小RNA-143 危险因素 无瘤生存 

分 类 号：R737.14[医药卫生—肿瘤] R699.5[医药卫生—临床医学]