甘遂中的新三萜及其抗氧化活性  

New triterpenes from roots of Euphorbia kansui and their antioxidant activities

作　　者：马丽 王嘉霖 柴甜 王铖博 孟宪华 杨军丽[1] MA Li;WANG Jia-lin;CHAI Tian;WANG Cheng-bo;MENG Xian-hua;YANG Jun-li(CAS Key Laboratory of Chemistry of Northwestern Plant Resources,Gansu Key Laboratory for Natural Medicine,Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences,Lanzhou 730000,China;University of Chinese Academy of Sciences,Beijing 100049,China;Northwest Minzu University,Lanzhou 730030,China)

机构地区：[1]中国科学院兰州化学物理研究所,中国科学院西北特色植物资源化学重点实验室,甘肃省天然药物重点实验室,甘肃兰州730000 [2]中国科学院大学,北京100049 [3]西北民族大学,甘肃兰州730030

出　　处：《中草药》2022年第8期2269-2273,共5页Chinese Traditional and Herbal Drugs

基　　金：甘肃省自然科学基金项目(21JR7RA075);甘肃省自然科学基金项目(20JR5RA570)。

摘　　要：目的 对甘遂Euphorbia kansui块根中的三萜化学成分和抗氧化活性进行研究。方法 采用多种柱色谱法进行分离纯化,并运用谱学分析鉴定化合物的结构。通过CCK-8法,对分离的化合物在SH-SY5Y细胞中的抗氧化活性进行了筛选。结果 从甘遂块根甲醇提取物中分离鉴定出5个三萜,其中包括3个大戟烷型,分别为大戟-8,24-二烯-3β-醇-7,11-二酮(1)、(24R)-大戟-8,25-二烯-3β,24-二醇(2)、大戟二烯醇(3);2个羊毛脂烷型三萜,3β,11β-二羟基羊毛脂-8,24-二烯-7-酮(4)、3β,7β-二羟基羊毛脂-8,24-二烯-11-酮(5)。结论 化合物5为新化合物,为羊毛脂烷型三萜,命名为甘遂羊毛脂酮A;化合物1和4为首次从该植物中分离得到;化合物2和5在SH-SY5Y细胞中具有一定的抗氧化能力和潜在的神经保护活性。Objective To isolate compounds from the roots of Euphorbia kansui and investigate their antioxidant activities.Methods Various column chromatography techniques were used to isolate and purify the compounds and their structures were identified by spectral data.Meanwhile, the isolated compounds were evaluated for antioxidant activity in SH-SY5Y cells.Results Five triterpenes were isolated and identified from the methanol extract of the roots of E.kansui, including three euphane triterpenes, namely eupha-8,24-diene-3β-ol-7,11-dione(1),(24R)-eupha-8,25-diene-3β,24-diol(2), euphol(3), and two lanostane triterpenes namely 3β,11β-dihydroxylanosta-8,24-dien-7-one(4), 3β,7β-dihydroxylanosta-8,24-dien-11-one(5).Conclusion Compound 5 is a new compound named euphokansuione A and compounds 1 and 4 are isolated from this plant for the first time.Compounds 2 and 5 have certain antioxidant capacity in SH-SY5Y cells and potential neuroprotective activity.

关 键 词：甘遂 三萜 大戟烷型三萜 羊毛脂烷型三萜 甘遂羊毛脂酮A 大戟二烯醇 抗氧化活性 神经保护活性 

分 类 号：R284.1[医药卫生—中药学]