益气散聚方对肥胖相关肾小球病模型小鼠AMPK信号通路的影响及机制研究  

Effect of Yiqi Sanju Recipe on AMPK signaling pathway of obesity-related glomerulopathy model mice and its mechanism

作　　者：方明珠[1,2] 徐艳秋 顾耀东[1] FANG Ming-zhu;XU Yan-qiu;GU Yao-dong(Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,CHINA;Department of Traditional Chinese Medicine,Jinshan Branch,Shanghai Sixth People's Hospital,Shanghai 201599,CHINA)

机构地区：[1]上海中医药大学附属岳阳中西医结合医院肾内科,上海200437 [2]上海市第六人民医院金山分院中医内科,上海201599

出　　处：《海南医学》2022年第9期1089-1095,共7页Hainan Medical Journal

基　　金：上海市卫生和计划生育委员会中医药科研项目(编号:2012JA001)。

摘　　要：目的探讨益气散聚方对肥胖相关性肾小球病模型小鼠体内AMPK与炎症诱发的关系以及对肾损害的影响,阐明益气散聚方防治肥胖相关性肾小球的作用机制。方法随机将6周龄的60只雄性C57BL/6试验小鼠分为正常组(6只)、西药组(12只)、中药组(18只)、中西医结合组(12只)、模型组(12只),正常组小鼠用生理盐水与普通饲料灌胃、西药组小鼠用二甲双胍与高脂饲料灌胃、中药组小鼠用益气散聚方与高脂饲料灌胃、中西医结合组小鼠用二甲双胍、益气散聚方与高脂饲料灌胃、模型组小鼠用生理盐水与高脂饲料灌胃。在药物干预的第4周末、第8周末观察各组小鼠的肥胖体质量,并检测24 h尿蛋白定量、尿RBP、尿NAG的变化情况,利用HE染色、免疫组化于镜下观察肾脏组织的病理变化,同时对肾脏组织内转化生长因子β_(1)蛋白(TGF-β_(1))、单核细胞趋化蛋白-1(MCP-1)、磷酸化腺苷酸活化蛋白激酶α(P-AMPKα)mRNA含量进行检测。结果在用药的第4周末和第8周末,正常组、中药组、模型组、西药组的小鼠体质量较前一次测量均增大,但差异无统计学意义(P>0.05);中西医结合组小鼠与其他各组相比体质量下降明显,其差异均具有统计学意义(P<0.05);在用药的第4周末和第8周末,中药组、中西医结合组、西药组与模型组相比,小鼠的24 h尿蛋白定量、尿RBP、尿NAG含量均降低,差异均具有显著统计学意义(P<0.01),且五组进行组间比较发现,中西医结合组与中药组的治疗效果较好,差异具有统计学意义(P<0.05);模型组、中药组、西药组、中西医结合组小鼠治疗的第4周末肾小球体积增大,肾小球囊腔变小,固有细胞无增生,系膜区未见明显增生,肾小管间质正常,未见细胞浸润;治疗第8周末检查发现与西药组、模型组进行比较,中西医结合组与中药组小鼠的肾小球直径均减小,但差异无统计学意义(P>0.05);药物干预�Objective To investigate the effect of Yiqi Sanju Recipe on AMPK signaling pathway,inflammation,renal damage in obesity-related glomerulopathy model mice,and to clarify the mechanism of Yiqi Sanju Recipe in the prevention and treatment of obesity-related glomerulopathy.Methods Sixty male C57BL/6 test mice at 6 weeks of age were randomly divided into normal group(n=6);western medicine group(n=12);traditional Chinese medicine group(n=18);integrated traditional Chinese and western medicine group(n=12);model group(n=12);which were gavaged with normal saline and common feed;metformin and high-fat feed;Yiqi Sanju Recipe and high-fat feed;metformin,Yiqi Sanju Recipe,and high-fat feed;normal saline and high-fat feed;respectively.At the end of the 4 th and 8 th week of treatment,the obesity body mass of mice in each group was observed,and the changes of 24 h urine protein,urine retinol-binding protein(RBP),and urine NAG were detected.The pathological changes of renal tissues were observed by HE staining and immunohistochemistry under microscope.Meanwhile,the mRNA levels of transforming growth factorβ_(1)(TGF-β_(1)),monocyte chemotaxis protein-1(McP-1),and phosphorylated adenylate activated protein kinaseα(P-AMPKα)in renal tissues were detected.Results At the end of the 4 th and 8 th week of treatment,the body mass of mice in normal group,traditional Chinese medicine group,model group,and western medicine group increased compared with the previous measurement,but the difference was not statistically significant(P>0.05);compared with other groups,the body mass of mice in the integrated traditional Chinese and western medicine group decreased significantly,and the difference was statistically significant(P<0.05).At the end of the 4 th and 8 th week of treatment,compared with the model group,the quantitative 24-h urinary protein,urinary RBP,and urinary NAG of mice in the traditional Chinese medicine group,integrated traditional Chinese and western medicine group,and western medicine group decreased,and the differences wer

关 键 词：C57BL/6试验小鼠 肥胖相关性肾病 益气散聚方 AMPK信号通路 炎症因子 

分 类 号：R-332[医药卫生]