去白细胞输血对急性白血病患者外周血Th1/Th2细胞平衡状况及医院感染发生率的影响  

Effects of leukocyte-depleted blood transfusion on peripheral blood Th1/Th2 cell balance and incidence of nosocomial infection in patients with acute leukemia

作　　者：李就文 李婉琴 陈昌达 宾科梅 LI Jiu-wen;LI Wan-qin;CHEN Chang-da;BIN Ke-mei(Department of Blood Transfusion,the Sixth Affiliated Hospital of Jinan University,Guangzhou 523573,Guangdong,CHINA;Department of Clinical Laboratory,the Sixth Affiliated Hospital of Jinan University,Guangzhou 523573,Guangdong,CHINA;Department of Hematology,the Sixth Affiliated Hospital of Jinan University,Guangzhou 523573,Guangdong,CHINA)

机构地区：[1]暨南大学附属第六医院输血科,广东广州523573 [2]暨南大学附属第六医院检验科,广东广州523573 [3]暨南大学附属第六医院血液科,广东广州523573

出　　处：《海南医学》2022年第9期1112-1115,共4页Hainan Medical Journal

摘　　要：目的探讨去白细胞输血对急性白血病患者外周血Th1/Th2细胞平衡状况及医院感染发生率的影响。方法选取2018年3月至2021年3月于暨南大学附属第六医院治疗的36例急性白血病患者为研究对象。依据随机单双数法分对照组和观察组各18例。对照组患者选择少白红细胞输血,观察组患者选择去白细胞输血。输血1周后,比较两组患者外周血Th1/Th2细胞平衡状况[白介素10(IL-10)、白介素2(IL-2)、白介素4(IL-4)、干扰素-γ(IFN-γ)、肿瘤坏死因子-α(TGF-α)]、T淋巴细胞CD3^(+)、CD4^(+)、CD8^(+)、CD4^(+)/CD8^(+)及医院感染发生情况。结果输血1周后,观察组患者的IL-10、IL-4、TGF-α水平分别为(26.76±5.99)ng/L、(81.83±12.06)ng/L、(17.49±3.49)pg/mL,明显低于对照组的(33.29±6.30)ng/L、(92.05±10.83)ng/L、(21.03±3.94)pg/mL,IL-2、IFN-γ分别为(38.84±7.36)ng/L、(33.18±5.12)ng/L,明显高于对照组的(31.53±8.41)ng/L、(28.52±5.22)ng/L,差异均有统计学意义(P<0.05);输血1周后,两组患者的CD3^(+)、CD4^(+)、CD8^(+)、CD4^(+)/CD8^(+)明显高于输血前,且观察组患者的CD3^(+)、CD4^(+)、CD8^(+)、CD4^(+)/CD8^(+)分别为(57.85±2.95)%、(31.03±1.73)%、(24.87±1.48)%、1.33±0.13,明显高于对照组的(53.22±2.41)%、(28.84±1.29)%、(23.26±1.46)%、1.19±0.11,差异均有统计学意义(P<0.05);观察组患者的医院感染发生率为33.33%,明显低于对照组的61.11%,差异有统计学意义(P<0.05)。结论去白细胞输血应用于急性白血病能使患者的外周血Th1/Th2细胞失衡状况得到显著改善,同时降低医院感染发生率,值得推广应用。Objective To investigate the effect of leukocyte-depleted blood transfusion on the balance of Th1/Th2 cells in peripheral blood and the incidence of nosocomial infection in patients with acute leukemia.Methods Thirty-six patients with acute leukemia who were treated in the Sixth Affiliated Hospital of Jinan University from March 2018 to March 2021 were selected as the research objects.According to random odd and even number method,the patients were divided into the control group and the observation group,with 18 patients in each group.The patients in the control group received leukocyte-reduced blood transfusion,while the patients in the observation group received leukocyte-depleted blood transfusion.After 1 week of blood transfusion,the balance of Th1/Th2 cells in the peripheral blood[including interleukin 10(IL-10),interleukin 2(IL-2),interleukin 4(IL-4),interferon-γ(IFN-γ),tumor necrosis factor-α(TGF-α)],T lymphocytes CD3^(+),CD4^(+),CD8^(+),CD4^(+)/CD8^(+),incidence of nosocomial infections of the two groups were compared.Results One week after blood transfusion,the levels of IL-10,IL-4,and TGF-αin the observation group were(26.76±5.99)ng/L,(81.83±12.06)ng/L,and(17.49±3.49)pg/mL,significantly lower than(33.29±6.30)ng/L,(92.05±10.83)ng/L,(21.03±3.94)pg/mL in the control group;IL-2 and IFN-γin the observation group were(38.84±7.36)ng/L and(33.18±5.12)ng/L,significantly higher than(31.53±8.41)ng/L and(28.52±5.22)ng/L in the control group;the differences were statistically significant(P<0.05).One week after blood transfusion,CD3^(+),CD4^(+),CD8^(+),CD4^(+)/CD8^(+)in the two groups were significantly higher than those before blood transfusion,and CD3^(+),CD4^(+),CD8^(+),CD4^(+)/CD8^(+)in the observation group were(57.85±2.95)%,(31.03±1.73)%,(24.87±1.48)%,1.33±0.13,significantly higher than(53.22±2.41)%,(28.84±1.29)%,(23.26±1.46)%,1.19±0.11 in the control group;the differences were statistically significant(P<0.05).The incidence of nosocomial infection in the observation group was 33.33%,whi

关 键 词：急性白血病 去白细胞输血 外周血Th1/Th2 细胞平衡 医院感染 T淋巴细胞 

分 类 号：R733.71[医药卫生—肿瘤]