两级A/O工艺处理焦化废水有机污染物转化特征及细菌群落响应  

Two-Stage Anoxic/Oxic(A/O)Process to Remove Organic Pollutants and Bacterial Community in Coking Wastewater

作　　者：邹晓爽 李江[1,2] 李彦澄[1,2] 王斌 易倩文[1] 张云涛 代永恒 ZOU Xiaoshuang;LI Jiang;LI Yancheng;WANG Bin;YI Qianwen;ZHANG Yuntao;DAI Yongheng(Key Laboratory of Karst Georesources and Environment,College of Resources and Environmental Engineering,Ministry of Education,Guizhou University,Guiyang 550025,China;Guizhou Karst Environmental Ecosystems Observation and Research Station,Ministry of Education,Guiyang 550025,China;College of Civil Engineering,Guizhou University,Guiyang 550025,China)

机构地区：[1]贵州大学资源与环境工程学院,喀斯特地质资源与环境教育部重点实验室,贵州贵阳550025 [2]贵州喀斯特环境生态系统教育部野外科学观测研究站,贵州贵阳550025 [3]贵州大学土木工程学院,贵州贵阳550025

出　　处：《环境科学研究》2022年第5期1245-1256,共12页Research of Environmental Sciences

基　　金：国家自然科学基金项目(No.51968008)。

摘　　要：为探究两级缺氧/好氧(anoxic/oxic,A/O)工艺处理焦化废水有机污染物转化和细菌群落结构之间的关系,以实际运行的焦化废水处理厂为研究对象,采用傅里叶变换红外光谱和三维荧光光谱对废水中有机污染物官能团与溶解性有机物(dissolved organic matter,DOM)进行分析,并采用高通量测序技术,分析两级A/O工艺中细菌群落结构及其功能的沿程变化.结果表明:①两级A/O工艺对化学需氧量(COD)、氨氮(NH_(4)^(+)-N)、硫氰酸盐(SCN-)、苯并[a]芘(BaP)、苯酚、挥发酚的去除率均在90%以上.②各有机污染物的去除与醇类、脂类、醚类、多糖类及羧酸类等物质的C≡N、C—N、C=C、—CO—伸缩振动,以及—NH_(2)和N—H弯曲振动等官能团运动有关,原水中大部分DOM被去除.③微生物分析表明,硫杆菌(Thiobacillus)为两级A/O工艺的优势菌属(相对丰度为18.85%~31.06%),Fluviicola和norank_f_JG30-KF-CM45的相对丰度与BaP浓度均呈负相关,Arenimonas的相对丰度与挥发酚、苯酚浓度均呈正相关,Bradyrhizobium、Nakamurella、Nitrospira、norank_f_NS9_marine_group、unclassified_f_Rhizobiaceae的相对丰度与挥发酚、苯酚浓度均呈负相关.④两级A/O工艺中有机污染物的去除与氟苯甲酸酯降解、多环芳烃降解、苯甲酸酯降解等多种代谢途径有关.研究显示,经两级A/O工艺处理后,焦化废水中的主要污染物均显著去除,并且有机污染物变化均会影响功能微生物相对丰度的改变.The coking wastewater with high concentration organic matter was treated by a two-stage anoxic/oxic(A/O)process to explore the relationship between the transformation of organic pollutants and bacterial community structure.Fourier transform infrared spectroscopy and three-dimensional fluorescence spectroscopy were used to analyze the functional groups of organic pollutants and dissolved organic matter in this two-stage A/O process.The structure and function of the microbial ecosystem were explored using high-throughput sequencing technology.The results showed the two-stage A/O process removed more than 90% of chemical oxygen demand from ammonia-borne nitrogen,thiocyanate,benzo[a]pyrene,phenol,and volatile phenols.The removal of each organic pollutant is related to shift in stretching vibrations of functional groups such as C≡N,C—N,C=C and —CO—,and bending vibrations of C—N and N—H.Such changes are applicable to alcohols,lipids,ethers,polysaccharides,and carboxylic acids.Additionally,most of DOM was removed from the original water.Microbial analysis showed Thiobacillus to be the dominant genus over the course of the treatment process(relative abundance ranging from 18.85% to 31.06%).Relative abundance of Fluviicola and Norank_F_JG30-KF-CM45 were negatively correlated with BaP concentration,while relative abundance of Arenimonas were positively correlated with the concentration of volatile phenol and phenol.Relative abundance of Bradyrhizobium,Nakamurella,Nitrospira,norank_f_NS9_marine_group and unclassified_f_Rhizobiaceae were negatively correlated with the concentration of volatile phenol and phenol.Furthermore,PICRUSt2 functional prediction analysis showed the removal of organic pollutants was associated with various metabolic pathways,such as the degradations of fluorobenzoate,PAH,and benzoate.The study showed that the main pollutants in coking wastewater were significantly removed by two-stage A/O process,and the change of organic pollutants affected the change of the relative abundance of function

关 键 词：焦化废水 官能团 胞外聚合物(EPS) 细菌群落结构 功能预测 

分 类 号：X172[环境科学与工程—环境科学]