构建一种新的细胞组织学评估模型探讨典型局灶性结节增生的中心瘢痕形成  

Central scar formation in classic focal nodular hyperplasia:A novel cytohistological evaluation model

作　　者：庄康敏[1] 林志昭[1] 孟琰[1] ZHAUNG Kang-min;LIN Zhi-zhao;MENG Yan(Guangdong Provincial Key Laboratory of Gastroenterology,Department of Gastroenterology,Nanfang Hospital,Southern Medical University,Guangzhou,Guangdong,510515 China)

机构地区：[1]广东省胃肠病学重点实验室,南方医科大学南方医院消化内科

出　　处：《现代消化及介入诊疗》2022年第2期164-168,共5页Modern Interventional Diagnosis and Treatment in Gastroenterology

基　　金：国家自然科学基金青年基金(81802965)。

摘　　要：目的探讨经典型肝局灶性结节状增生(FNH)中央瘢痕的细胞组织学机制。方法从手术切除的具有中央瘢痕的FNH标本中分离出活化的肝星状细胞。采用细胞免疫荧光法及定量逆转录聚合酶链反应检测特定的细胞骨架标记物,如α-SMA、Collagen l和MMP-2。同时,收集有或无中央瘢痕的28例FNH样本,采用免疫组织化学染色和双免疫荧光标记法检测α-SMA、Collagen l的表达情况。结果FNH标本分离的肝星状细胞在培养期间具有肌成纤维细胞样的形态,同时高表达desmin,α-SMA,collagen I and MMP-2。同时,在FNH组织中可检测到中央瘢痕区域α-SMA,collagen I蛋白的共表达,且与组织大小显著相关。结论本研究首次基于细胞学-组织学水平上探讨FNH组织中央瘢痕形成的潜在机制,研究结果可为经典型FNH及中央瘢痕的形成提供实验资料。Objective To investigate the cellular and histological mechanism of central scar in classical focal nodular hyperplasia of the liver(FNH).Methods Activated hepatic stellate cells(HSCs)were isolated from surgically resected specimens of FNH with a central scar.Cell immunofluorescence were used to detect the specific cytoskeletal markers,such as desmin,α-smooth muscle actin(α-SMA),type I collagen and matrix metalloprotease-2(MMP-2).Further histological researches about immunohistochemical staining and double immunofluorescence labeling method ofα-SMA and type I collagen were detected in 28 FNH samples with or without central scars.Results FNH isolated-HSCs shared a myofibroblast-like morphology during culture and expressed strong level of desmin,α-SMA,type I collagen and MMP-2.Protein levels ofα-SMA,type I collagen were were detected in classic FNH with co-localization and expressed differently from the size of lesions.Conclusion This study is the first to explore the potential mechanism of central scar formation in FNH based on the level of cytology and histology.The results can provide experimental data for the formation of classical FNH and central scar.

关 键 词：肝局灶性结节状增生 中央瘢痕 肝星状细胞 细胞骨架蛋白 

分 类 号：R575[医药卫生—消化系统]