miR-3189-3p在肾癌组织中的表达及对肾癌细胞增殖和侵袭的影响  

Expression of miR-3189-3p in renal carcinoma and its effect on the proliferation and invasion of renal carcinoma cells

作　　者：曾光 胡晓晖 杨超 廖兆琳 杜然 Zeng Guang;Hu Xiaohui;Yang Chao;Liao Zhaolin;Du Ran(Department of Urology,the Central Hospital of Enshi Tujia and Miao Autonomous Prefecture,Enshi 445000,China;Department of Urology,the National Hospital of Enshi Tujia and Miao Autonomous Prefecture,Enshi 445000,China;Department of Nephrology,the Central Hospital of Enshi Tujia and Miao Autonomous Prefecture,Enshi 445000,China)

机构地区：[1]恩施土家族苗族自治州中心医院泌尿外科,恩施445000 [2]恩施土家族苗族自治州民族医院泌尿外科,恩施445000 [3]恩施土家族苗族自治州中心医院肾病科,恩施445000

出　　处：《国际外科学杂志》2022年第3期202-206,I0007,共6页International Journal of Surgery

基　　金：湖北省卫生计生委中医药科研面上项目(ZY2019M077)。

摘　　要：目的探讨miR-3189-3p在肾癌组织和癌旁组织中的表达差异及其对肾癌细胞生物学功能的影响。方法通过实时荧光定量聚合酶链反应(qRT-PCR)检测miR-3189-3p在肾癌组织及癌旁组织、肾癌细胞株(Caki-1、ACHN、A498、OS-RC-2)和正常肾小管上皮细胞HK-2中的表达。分别转染miR-NC或miR-3189-3p mimics至miR-3189-3p表达最低的肾癌细胞,分别为miR-NC组和miR-3189-3p组。通过CCK-8法和Transwell迁移实验分别检测miR-3189-3p对肾癌细胞增殖和侵袭的影响。miRanda和miRTarBase软件预测miR-3189-3p的下游基因。双荧光素酶报告基因实验验证miR-3189-3p的下游基因。qRT-PCR和Western blotting法检测miR-3189-3p下游基因的表达。计量资料以均数±标准差(±s)表示,组间比较采用t检验。结果miR-3189-3p在肾癌组织和癌旁组织中的相对表达量分别为1.97±0.61和6.19±0.73,miR-3189-3p在肾癌组织的相对表达量低于癌旁组织(P<0.01)。miR-3189-3p在肾癌细胞株的相对表达量均低于HK-2细胞(P<0.05),OS-RC-2细胞中相对表达量最低(P<0.01)。miR-NC组和miR-3189-3p组OS-RC-2细胞中miR-3189-3p的相对表达量分别为1.01±0.11和9.27±1.43,miR-NC组miR-3189-3p的相对表达量明显低于miR-3189-3p组(P<0.01)。与miR-NC组相比,高表达miR-3189-3p的OS-RC-2细胞增殖能力明显降低(P<0.05)。miR-NC组和miR-3189-3p组穿膜细胞数分别为(165.40±17.02)个和(41.07±6.36)个,miR-3189-3p组OS-RC-2细胞侵袭能力明显降低(P<0.01)。miR-3189-3p的靶基因是水通道蛋白3(AQP3),miR-3189-3p可靶向结合AQP3 mRNA(P<0.01)。与miR-NC组相比,高表达miR-3189-3p细胞中AQP3基因在mRNA水平和蛋白水平的表达均明显降低(P<0.01)。结论miR-3189-3p在肾癌中表达下调,高表达miR-3189-3p可显著抑制肾癌OS-RC-2细胞的增殖和侵袭,其分子机制是miR-3189-3p靶向抑制AQP3基因的表达。Objective:To investigate the expression difference of miR-3189-3p in renal cancer tissue and adjacent tissue and its effect on the biological function of renal cancer cells.Methods:quantitative real-time polymerase chain reaction(qRT-PCR)was used to detect the expression level of miR-3189-3p in renal cancer tissues and adjacent tissues,renal cancer cell lines(Caki-1,ACHN,A498,OS-RC-2)and normal renal tubular epithelial cells HK-2.miR-NC or miR-3189-3p mimics were transfected into renal cancer cells with the lowest expression of miR-3189-3p,respectively,named miR-NC group and miR-3189-3p group.The effects of miR-3189-3p on the proliferation and invasion of renal cancer cells were detected by CCK-8 method and Transwell migration experiment.miRanda and miRTarBase software was used to predict the downstream gene of miR-3189-3p.The dual luciferase reporter gene experiment was used to verify the downstream gene of miR-3189-3p.qRT-PCR and Western blotting were used to detect the expression of miR-3189-3p downstream gene.Measurement data were expressed as mean±standard deviation(±s),and t-test was used for comparison between groups.Results:The relative expression of miR-3189-3p in renal cancer tissue and paracancerous tissue was 1.97±0.61 and 6.19±0.73,respectively,and the relative expression of miR-3189-3p in renal cancer tissue was lower than that in paracancerous tissue(P<0.01).The relative expression of miR-3189-3p in renal cancer cell lines was lower than that in HK-2 cells(P<0.05).The relative expression of miR-3189-3p in OS-RC-2 cells was the lowest(P<0.01).The relative expression levels of miR-3189-3p in OS-RC-2 cells in the miR-NC group and miR-3189-3p group were 1.01±0.11 and 9.27±1.43,respectively,and the relative expression levels of miR-3189-3p in the miR-NC group significantly lower than the miR-3189-3p group(P<0.01).Compared with the miR-NC group,the proliferation ability of OS-RC-2 cells with high expression of miR-3189-3p was significantly reduced(P<0.05).The numbers of penetrating cells in the miR

关 键 词：肾肿瘤 miR-3189-3p 水通道蛋白3 细胞增殖 细胞侵袭 

分 类 号：R737.11[医药卫生—肿瘤]