单环刺螠体腔液抑菌特性研究  

Antibacterial Analysis of Coelomic Fluid from Echiuran Worm Urechis unicinctus

作　　者：甘宏涛 刘统昊 丁子媛 姚海洋 崔楠 孟霄 徐国成 曹黎鑫 许星鸿 GAN Hongtao;LIU Tonghao;DING Ziyuan;YAO Haiyang;CUI Nan;MENG Xiao;XU Guocheng;CAO Lixin;XU Xinghong(College of Marine Life and Fisheries, Jiangsu Ocean University, Lianyungang 222005, China)

机构地区：[1]江苏海洋大学海洋生命与水产学院,江苏连云港222005

出　　处：《水产科学》2022年第3期459-466,共8页Fisheries Science

基　　金：江苏省科技厅苏北科技专项(LYG-SZ201814);江苏省优势学科建设工程资助项目(5511201901X);江苏省研究生和大学生科研与实践创新计划项目(KYCX19_2287);连云港市科技局现代农业项目(CN1411);连云港521高层次人才培养工程项目(KK18027).

摘　　要：自体质量(18.03±1.35)g单环刺螠中抽取体腔液,于4℃、3000 r/min(离心半径13.5 cm)离心20 min,取体腔液上清液,再将离心所得血细胞沉淀用无菌生理盐水重悬后,经超声波破碎仪4℃破碎5 min,取体腔细胞破碎液上清液,以牛津杯法和生长曲线抑制法检测单环刺螠体腔液上清液和体腔细胞破碎液上清液对嗜水气单胞菌、大肠杆菌、副溶血弧菌、鳗弧菌、凡隆氏气单胞菌、哈维氏弧菌、金黄色葡萄球菌和溶壁微球菌8种菌的抑制效果,并分析温度、盐度、干露和饥饿等环境因子对体腔液抑菌活性的影响。试验结果显示,单环刺螠体腔液上清液对鳗弧菌和副溶血弧菌具有显著抑制作用(P<0.05),体腔细胞破碎液上清液对鳗弧菌、副溶血弧菌和凡隆氏气单胞菌具有显著抑制作用(P<0.05)。盐度25组体腔细胞破碎液上清液抑菌活性较强,盐度20和30两组抑菌活性略低,低盐胁迫(盐度15)和高盐胁迫(盐度35)下,抑菌活性下降极显著(P<0.01),以低盐胁迫影响更大。当温度处于6~12℃,体腔细胞破碎液上清液抑菌活性较强,低于12℃或高于26℃,抑菌活性均发生极显著下降(P<0.01)。干露时间≥8 h,体腔液抑菌活性显著降低(P<0.05);饥饿时间≥2 d,体腔液抑菌活性下降极显著(P<0.01)。The coelomic fluid of echiuran worm Urechis unicinctus with body weight of(18.03±1.35)g was centrifuged at 4℃,3000 r/min(centrifugal radius of 13.5 cm)for 20 min,and the supernatant was taken,and then centrifuged the blood cell pellet by centrifugation.After resuspending in sterile normal saline,it was crushed by ultrasonic disintegrator at 4℃for 5 min,and the supernatant was taken as the supernatant of the coelomic cell crushing liquid.The antibacterial effects of the coelomic fluid supernatant,and coelomic cell crushing liquid on Aeromonas hydrophila,Escherichia coli,Vibrio parahemolyticus,V.anguillarum,A.veronii,V.harveyi,Staphylococcus aureus,and Micrococcus lysodeikticus were determined by the methods of Oxford cup and bacterial growth curve determination.Meanwhile,the influence of environmental factors including temperature,salinity,air exposure and fast on the antibacterial activity of coelomic fluid of the echiuran worm were investigated.The results showed that coelomic fluid supernatant had significant inhibiton on V.anguillarum and V.parahaemolyticus(P<0.05),while the coelomocyte ocytelysate supernatant had a significant bacteriostatic effect on V.anguillarum,V.parahaemolyticus and A.fragilis(P<0.05).There was slightly higher significant inhibition in the supernatant of the coelomic cell crushing liquid in the treatment group of salinity 25 than that in salinity 20 and 30 groups.However,the antibacterial activity of the coelomic cell crushing supernatant was found to be decreased significantly at salinity of 15 and 35,low salinity(15)showing more significant effect than at high salinity(35)(P<0.01).The coelomic cell crushing supernatant led to higher bacteriostatic effect under the temperature of 22—26℃,while the inhibition effect was declined significantly with the temperature below than 12℃or more than 26℃(P<0.01).The significantly lower antibacterial activity was observed in the coelomic fluid of echiuran worm exposed to the stress of air exposure for 8 h or more(P<0.05),and to be fasten

关 键 词：单环刺螠 体腔液 抑菌活性 环境胁迫 

分 类 号：S917[农业科学—水产科学]