Jumonji结构域蛋白3调节人牙周膜细胞的炎症反应  

Jumonji Domain-containing Protein 3 Regulates the Inflammatory Response of Human Periodontal Ligament Cells

作　　者：王璞玉 索来 WANG Puyu;SUO Lai(Department Ⅱ of Endodontics, Tianjin Stomatological Hospital, Tianjin 300041, China;Department of International VIP Dental Clinic, Tianjin Stomatological Hospital, Tianjin 300041, China)

机构地区：[1]天津市口腔医院牙体牙髓二科,天津300041 [2]天津市口腔医院国际诊疗中心,天津300041

出　　处：《口腔医学研究》2022年第5期424-428,共5页Journal of Oral Science Research

基　　金：天津市口腔医院科技基金(青年项目)(编号:2019BSQN01、2019BSQN02);天津市卫健委青年项目(编号:TJWJ2021QN066);天津市医学重点学科(专科)建设项目。

摘　　要：目的:研究三甲基化组蛋白H3赖氨酸K27(histone 3 lysine 27 trimethylation,H3K27me3)的去甲基化酶Jumonji结构域蛋白3(Jumonji domain-containing protein 3,Jmjd3)在人牙周膜细胞炎症反应中的调节作用。方法:分别使用牙龈卟啉单胞菌(Porphyromonas gingivalis,p.g)的脂多糖(lipopolysaccharide,LPS)和肿瘤坏死因子肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)刺激人牙周膜细胞,建立炎症模型。通过实时荧光定量聚合酶链反应(quantitative real-time polymerase chain reaction,Q-PCR)和免疫荧光检测Jmjd3的表达情况。后通过沉默Jmjd3,在Q-PCR和Western blot确定沉默效果后对其可能的下游靶基因进行初步筛选。结果:Jmjd3在炎症的人牙周膜细胞中表达会上调。沉默Jmjd3后,炎症介导的白细胞介素-6和白细胞介素-12b的上调受到抑制。结论:Jmjd3参与人牙周膜细胞炎症反应,并能够调节白细胞介素-6、白细胞介素-12b的表达。Objective:To investigate the role of the histone 3 lysine 27 trimethylation(H3K27me3)demethylase Jumonji domain-containing protein 3(Jmjd3)in the regulation of the inflammatory response of human periodontal ligament cells(HPDLs).Methods:HPDLs were stimulated with lipopolysaccharide(LPS)from p.g or tumor necrosis factor-α.The expression of Jmjd3 in HPDLs was examined by quantitative real-time polymerase chain reaction(Q-PCR)and immunofluorescence.After confirmed the depletion of Jmjd3 by Q-PCR and Western blot,potential target genes were selected by silencing Jmjd3.Results:Q-PCR and immunofluorescence revealed that the expression of Jmjd3 was increased in inflamed HPDLs.Knockdown of Jmjd3 led to the suppression of inflammation-induced up-regulation of interleukin-6 and interleukin-12b.Conclusion:Jmjd3 regulated the activation of interleukin-6 and interleukin-12b in the inflammatory response of HPDLs.

关 键 词：Jumonji结构域蛋白3 牙周膜细胞 炎症反应 

分 类 号：R78[医药卫生—口腔医学]