纳米银胁迫对汤氏纺锤水蚤的生殖及基因毒性效应研究  

Reproductive and Genotoxic Effects of Silver Nanoparticles Exposure on Acartia tonsa

作　　者：刘星辰 吕泓玥 金扬湖 周超 LIU Xing-chen;LYU Hong-yue;JIN Yang-hu(School of Marine Science and Technology of Zhejiang Ocean University,National Engineering Research Center For Marine Aquaculture,Zhoushan 316022,China)

机构地区：[1]浙江海洋大学海洋科学与技术学院,国家海洋设施养殖工程技术研究中心,浙江舟山316022

出　　处：《浙江海洋大学学报（自然科学版）》2022年第2期156-162,共7页Journal of Zhejiang Ocean University：Natural Science

基　　金：浙江省自然科学基金青年科学基金项目(LQ18D060006);舟山市科技局“浙江海洋大学科技专项”(2019C21095);国家级大学生创新创业训练计划创新训练项目(201810340004);国家自然科学基金国际(地区)合作研究项目(42020104009)。

摘　　要：为探究海洋毒性监测模式物种-汤氏纺锤水蚤对新型环境污染物纳米银的毒性响应机制,本文研究纳米银暴露后各个时间段对水蚤产卵量、卵孵化率、排便量、超氧化物歧化酶酶活以及水蚤Hsp70及Ferritin基因表达水平影响。纳米银作用下汤氏纺锤水蚤急性及半慢性EC_(50)分别为0.67 mg·L^(-1)、0.2 mg·L^(-1)。根据半慢性EC_(50)进行纳米银慢性毒理试验,研究发现汤氏纺锤水蚤产卵量、孵化率及排便量会呈现持续下降趋势。纳米银胁迫初期对水蚤机体造成损伤,与对照组相比,SOD酶活、Ferritin及Hsp70基因均有显著低量表达(P<0.05),但纳米银毒性积累至一定量后,可能会造成不可逆的损伤,表现为SOD酶活、Ferritin及Hsp70基因表达量随着时间增加呈现先上升后下降的趋势。因此,汤氏纺锤水蚤对纳米银胁迫响应具有浓度效应以及时间效应。In order to explore the toxic response mechanism of marine copepod Acartia tonsa,which exposed to silver nanoparticles in a temperate model,the effects of nano silver on the egg production,egg hatching rate,defecation volume,superoxide dismutase activity and Hsp70 and Ferritin gene expression levels were studied.The acute and semi chronic EC50 were 0.67 mg·L^(-1)and 0.2 mg·L^(-1),respectively.Chronic toxicological tests of silver nanoparticles were carried out according to the semi chronic EC50.Compared with the control group,the expression of SOD enzyme activity,Ferritin and Hsp70 genes were significantly lower(P<0.05),but after the nano silver toxicity accumulated to a certain amount,it may cause the irreversible damage manifested as SOD enzyme activity,Ferritin and Hsp70 gene expression increased first and then decreased with time.Therefore,there were concentration effect and time effect in response to nano silver stress.

关 键 词：纳米银 汤氏纺锤水蚤 行为学 酶活 基因表达 

分 类 号：Q89[生物学] X171.5[环境科学与工程—环境科学]