壳聚糖-纳米ZnO-褪黑素复合涂膜对黄瓜冷害的影响及其机制研究  

Effect of chitosan-nano-ZnO-melatonin composite coating on cucumber chilling injury and mechanism

作　　者：王锋[1] 赵旗峰 张晓萍 杨青珍[1] WANG Feng;ZHAO Qifeng;ZHANG Xiaoping;YANG Qingzhen(Department of Life Sciences,Yuncheng University,Yuncheng 044000,China;Pomology Institute of Shanxi Agricultural University,Taigu 030800,China)

机构地区：[1]运城学院生命科学系,山西运城044000 [2]山西农业大学果树研究所,山西太谷030800

出　　处：《食品与发酵工业》2022年第9期201-207,共7页Food and Fermentation Industries

基　　金：博士基金启动项目(YQ-2016013);食品科学与工程重点学科项目(XK-2021012,XK-2021015);山西省重点学科群建设项目(FSKSC)。

摘　　要：为探究涂膜处理对黄瓜冷藏期间冷害的影响,对壳聚糖-纳米ZnO、褪黑素及壳聚糖-纳米ZnO-褪黑素复合涂膜处理黄瓜的冷害指数、冷害率、失重率、腐烂率、呼吸速率、硬度、抗坏血酸含量、抗氧化酶[超氧化物歧化酶(superoxide dismutase,SOD)、抗坏血酸过氧化物酶(ascorbate peroxidase,APX)、过氧化氢酶(catalase,CAT)和谷胱甘肽还原酶(glutathione reductase,GR)]活性、超氧阴离子自由基(·O_(2)^(-))生成速率、H_(2)O_(2)含量、丙二醛(malondialdehyde,MDA)含量和细胞膜相对透性进行定期检测和分析。结果表明,壳聚糖-纳米ZnO、褪黑素及壳聚糖-纳米ZnO-褪黑素复合涂膜有效降低冷藏黄瓜冷害指数和冷害率,保持较低的失重率和腐烂率,延缓呼吸速率上升,并显著抑制硬度和抗坏血酸含量的下降;保持较高的SOD、APX、CAT和GR活性,显著降低·O_(2)^(-)生成速率和H_(2)O_(2)含量,抑制MDA积累和细胞膜相对透性上升。这些结果表明,3种处理均可减轻黄瓜冷害,延长其贮藏期,其中以壳聚糖-纳米ZnO-褪黑素复合涂膜处理效果最佳。该研究结果为减轻黄瓜冷害提供了一定的理论依据。To investigate the effect of coating on chilling injury of cucumber during low-temperature storage,the following indicators were regularly detected and analyzed,which including chilling injury index,chilling injury rate,weight loss rate,decay rate,respiration rate,firmness,ascorbic acid content,antioxidant enzymes activities [superoxide dismutase(SOD),ascorbate peroxidase(APX),catalase(CAT) and glutathione reductase(GR)],superoxide anion production(·O_(2)^(-)),H_(2)O_(2) content,malondialdehyde(MDA) accumulation and membrane relative permeability of chitosan-nano-ZnO,melatonin and chitosan-nano-ZnO-melatonin composite.The results showed that chitosan-nano-ZnO,melatonin and chitosan-nano-zinc oxide-melatonin composite coating could significantly reduce the chilling injury index and chilling injury rate,maintain low weight loss rate and decay rate and delay respiration rate.Meanwhile,it also significantly inhibited the decrease of firmness and ascorbic acid content and increased the activities of SOD,APX,CAT and GR.Besides,the rate of ·O_(2)^(-) production and H_(2)O_(2) content were also reduced and MDA accumulation and membrane relative permeability were delayed.These results showed that above three treatments could reduce chilling injury and prolong the storage period of cucumber,and chitosan-nano-ZnO-melatonin composite coating had the best effect.This study will provide a theoretical basis for alleviation of chilling injury of cucumber.

关 键 词：黄瓜 冷害 复合涂膜 品质 抗氧化酶 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]