一株鸡源植物乳杆菌益生性能的评价  

Evaluation of probiotics of lactobacillus plantarum isolated from chicken

作　　者：刘震坤 陈鲜鑫[1] 任思宇 李世易[1] LIU Zhenkun;CHEN Xianxin;REN Siyu;LI Shiyi(Leshan Institute of Agricultural Sciences.Leshan,Sichuan Province 61400,China;Chongqing Three Gorges Vocational College,Chongqing 404155,China)

机构地区：[1]乐山市农业科学研究院,四川乐山614000 [2]重庆三峡职业学院,重庆万州404155

出　　处：《中国饲料》2022年第8期21-24,共4页China Feed

基　　金：四川省科技计划项目(2020NZZJ029);四川省科技计划资助(2021JDRC0142);重庆市教育委员会科学技术研究项目(KJQN201803501);乐山市科技成果转化项目(20CGZH001)。

摘　　要：为评价一株来源于鸡肠道内容物的植物乳杆菌DK106的益生性能,试验测定了该菌株的生长特点、抗逆性、药物敏感性、抗氧化能力和黏附性等。结果显示,该菌株在14 h进入生长稳定期;能耐受0.3%胆盐浓度;对氟苯尼考、头孢噻肟、头孢曲松和林可霉素敏感;DPPH自由基清除率高达90.79%;该菌株经二甲苯处理30 min后,其表面疏水率高达97.40%;自凝聚率在第5小时可达68.80%;与大肠杆菌、沙门菌、金黄色葡萄球菌的第2小时共凝聚率分别达到20.59%、16.37%和55.32%。综上所述,植物乳杆菌DK106具有良好的益生性能,可作为家禽微生物饲料添加剂的菌株来源。In order to evaluate the probiotics of Lactobacillus plantarum DK106 from chicken intestinal contents,the growth characteristics,stress resistance,protease producing ability,drug sensitivity,anti-oxidation ability and adhesion of the strain were tested.The results showed that plantarum DK106 entered the stable period of growth at 14h.The strain could tolerate 0.3%bile salt concentration.It was sensitive to Florfenicol,Cefotaxime,Ceftriaxone and Lincomycin.and DPPH free radical scavenging rate was as high as 90.79%.The surface hydrophobicity was 97.40%after treated with xylene for 30 min.Its auto-aggregation ratio rate was 68.80%at 5h,and the co-aggregation ratios were 20.59%,16.37%and 55.32%with E.coli,salmonella and Staphylococcus Aureus,respectively.In conclusion,lactobacillus plantarum DK106 had good probiotic properties and could be used as a strain source of microbial feed additive for poultry.

关 键 词：鸡 植物乳杆菌 益生性能 

分 类 号：S831[农业科学—畜牧学]