三维CT定量及定性参数与纯磨玻璃结节样肺腺癌TOP2A、BASP1、FAM72蛋白表达的相关性研究  

Correlation between quantitative and qualitative parameters of three-dimensional CT and protein expression of TOP2A,BASP1 and FAM72 in lung adenocarcinoma presented as pure ground glass nodular

作　　者：许双燕 冯涛 曹宏伟[1] 曹振东[1] XU Shuang-yan;FENG Tao;CAO Hong-wei(Department of Radiology,Affiliated Hospital of Chengde Medical College,Hebei 067000,China)

机构地区：[1]河北省承德市承德医学院附属医院放射科,河北067000

出　　处：《放射学实践》2022年第5期543-549,共7页Radiologic Practice

摘　　要：目的:探讨纯磨玻璃结节(pGGN)样肺腺癌的三维CT定量及定性参数与拓扑异构酶2α(TOP2A)、脑酸溶性蛋白1(BASP1)、序列相似家族72成员(FAM72)蛋白表达间的相关性。方法:纳入行手术治疗的164例pGGN样肺腺癌患者作为研究对象,所有患者均行胸部CT扫描,记录结节最长径、结节体积、平均CT值及是否出现分叶征、空泡征、血管集束征、充气支气管征、胸膜牵拉征和瘤肺界面模糊征。肺腺癌组织中的TOP2A、BASP1、FAM72蛋白表达水平采用免疫组织化学法进行测定。结果:164例肺腺癌患者中,TOP2A高表达94例(57.3%),低表达70例(42.7%);BASP1高表达88例(53.7%),低表达76例(46.3%);FAM72高表达96例(58.5%),低表达68例(41.5%)。单因素分析结果显示,TOP2A的表达水平与三维CT定量指标中的结节最长径、结节体积和结节平均CT值相关(P<0.05),与定性指标中的分叶征、血管集束征和充气支气管征有关(P<0.05);BASP1的表达水平与三维CT定量指标中的结节最长径和结节平均CT值有关(P<0.05),与定性指标中的分叶征、血管集束征、充气支气管征和胸膜牵拉征有关(P<0.05);FAM72的表达水平与三维CT定量指标中的结节平均CT值有关(P<0.05),与定性指标中的血管集束征、充气支气管征和胸膜牵拉征有关(P<0.05)。多因素二元Logistic分析结果显示,结节最长径、结节平均CT值和血管集束征是肺腺癌TOP2A高表达的独立预测因子(P<0.05),结节最长径、结节平均CT值、血管集束征和胸膜牵拉征是BASP1高表达的独立预测因子(P<0.05),结节平均CT值、血管集束征和充气支气管征是肺腺癌FAM72高表达的独立预测因子(P<0.05)。结论:三维CT定量及定性参数与pGGN肺腺癌TOP2A、BASP1、FAM72蛋白表达水平相关,有助于预测患者预后和制定临床治疗方案。Objective:To investigate the correlation between the quantitative and qualitative parameters of three-dimensional(3D)CT and the protein expression of topoisomerase 2α(TOP2A),brain acid-soluble protein 1(BASP1)and 72 members of sequence similar family(FAM72)in lung adenocarcinoma presented as pure ground glass nodules(pGGN).Methods:A total of 164 patients with lung adenocarcinoma presented as pGGN who received surgical treatment were included in this study.All patients underwent chest CT scanning,and the longest diameter,volume,average CT value,lobulation sign,vacuole sign,vascular cluster sign,air-filled bronchial sign,pleural traction sign and ambiguous tumor-lung interface sign were recorded.The protein expression levels of TOP2A,BASP1 and FAM72 in lung adenocarcinoma were determined by immunohistochemistry and recorded.Results:Among 164 patients,94(57.3%)had high expression of TOP2A and 70(42.7%)had low expression of TOP2A;88(53.7%)had high expression of BASP1 and 76(46.3%)had low expression of BASP1;96(58.5%)had high expression of FAM72 and 68(41.5%)had low expression of FAM72.Univariate analysis showed that the expression level of TOP2A was correlated with the longest diameter,volume and average CT value of the nodules in the quantitative indicators of 3D CT(P<0.05),and was correlated with the lobulation sign,vascular cluster sign and air-filled bronchial sign in the qualitative indicators(P<0.05).The expression level of BASP1 was correlated with the longest diameter and average CT value of the nodules in the quantitative indicators of 3D CT(P<0.05),and was correlated with the lobulation sign,vascular cluster sign,air-filled bronchial sign and pleural traction sign in the qualitative indicators(P<0.05).The expression level of FAM72 was correlated with the average CT value of nodules in the quantitative indicators of 3D CT(P<0.05),and was correlated with the vascular cluster sign,air-filled bronchial sign and pleural traction sign in the qualitative indicators(P<0.05).Multivariate binary logistic analysis sh

关 键 词：肺腺癌 磨玻璃结节 体层摄影术 X线计算机 TOP2A BASP1 FAM72 

分 类 号：R734.2[医药卫生—肿瘤] R814.42[医药卫生—临床医学]