Q开关Nd:YAG 1064 nm激光对糠秕马拉色菌细胞活性、蛋白酶活性及结构的影响  

Effect of 1 064-nm Q-switched Nd:YAG laser on cell viability, protease activity and structures of Malassezia furfur

作　　者：谈桂其 钟静 张丽丹 李晓辉[1] 彭洁雯[1] 翁智胜[1] Tan Guiqi;Zhong Jing;Zhang Lidan;Li Xiaohui;Peng Jiewen;Weng Zhisheng(Department of Dermatology,The Third Affiliated Hospital of Guangzhou Medical University,Guangzhou 510150,China)

机构地区：[1]广州医科大学附属第三医院皮肤科,广州510150

出　　处：《中华皮肤科杂志》2022年第5期425-429,共5页Chinese Journal of Dermatology

摘　　要：目的探讨不同能量Q开关Nd:YAG 1064 nm激光对糠秕马拉色菌的细胞活性、蛋白酶活性及菌体结构的影响。方法选用糠秕马拉色菌标准株,分别予以Q开关Nd:YAG 1064 nm激光0(对照组)、500、600、700、800、900 mJ能量照射,培养7 d后测量各组菌落直径及菌落数,评估菌体细胞活性,采用全脂牛奶平板法测定蛋白酶活性,透射电镜观察各组菌体超微结构。多组间比较采用单因素方差分析,组间两两比较采用LSD-t检验,激光能量与菌落直径、菌落数及蛋白酶活性的相关性采用Pearson相关分析。结果对照组及500、600、700、800、900 mJ组菌落直径(4.05±0.69、3.76±0.51、3.28±0.41、3.09±0.72、2.54±0.64、2.43±0.41 mm)、菌落数(4787±597、4287±761、1879±275、1082±248、209±42、72±31)差异均有统计学意义(F值分别为14.83、231.85,P<0.05),600、700、800、900 mJ组菌落直径、菌落数均小于对照组(均P<0.05)。激光能量与菌落直径和菌落数均呈负相关(r值分别为-0.67、-0.91,P<0.05)。对照组、500 mJ组、700 mJ组、900 mJ组蛋白酶活性差异有统计学意义(F=346.60,P<0.05),700 mJ、900 mJ组蛋白酶活性均低于对照组(P<0.05)。激光能量与蛋白酶活性呈负相关(r=-0.94,P<0.05)。透射电镜下观察到对照组菌体结构完整,500 mJ组菌体结构较完整,600~900 mJ组结构明显受破坏,且激光能量越大菌体结构破坏越严重。结论Q开关Nd:YAG 1064 nm激光可影响糠秕马拉色菌细胞及蛋白酶活性,破坏菌体结构。Objective To investigate the effect of 1064-nm Q-switched Nd:YAG laser at different energy settings on cell viability,protease activity and structures of Malassezia furfur.Methods Cultured standard strains of Malassezia furfur were divided into several groups to be irradiated with 1064-nm Q-switched Nd:YAG laser at different energies of 0(control group),500,600,700,800 and 900 mJ,respectively.Then,fungal suspensions in the above groups were inoculated onto the Leeming&Notman medium separately.After 7-day culture,the diameter and number of colonies were measured to evaluate the fungal cell viability,the protease activity was measured by using the whole-milk plate medium,and the ultrastructure of Malassezia furfur in each group was observed by transmission electron microscopy.One-way analysis of variance was used for comparisons among multiple groups,least significant difference-t test for multiple comparisons,and Pearson correlation analysis for analyzing correlations of laser energy with colony diameter,colony number and protease activity.Results The colony diameter and number both significantly differed among the control group,500-,600-,700-,800-and 900-mJ groups(colony diameter:4.05±0.69,3.76±0.51,3.28±0.41,3.09±0.72,2.54±0.64 and 2.43±0.41 mm,respectively;colony number:4787±597,4287±761,1879±275,1082±248 and 209±42,72±31 colony-forming units,respectively;F=14.83,231.85,respectively,both P<0.05),and were significantly lower in the 600-,700-,800-and 900-mJ groups than in the control group(all P<0.05).The laser energy was negatively correlated with the colony diameter and number(r=-0.67,-0.91,respectively,both P<0.05).The protease activity significantly differed among the control group,500-,700-and 900-mJ groups(F=346.60,P<0.05),and was significantly lower in the 700-and 900-mJ groups than in the control group(both P<0.05).There was a negative correlation between the laser energy and protease activity(r=-0.94,P<0.05).Transmission electron microscopy showed intact fungal structures in the control group

关 键 词：马拉色霉菌属 激光 固体 ND-YAG激光 细胞结构 细胞活性 蛋白酶活性 

分 类 号：R756[医药卫生—皮肤病学与性病学]