血管生成素1在非小细胞肺癌中的表达及其对肺癌细胞生物学行为的影响  

Expression of angiopoietin 1 in non-small cell lung cancer and its effect on the biological behavior of lung cancer cells

作　　者：王继振 刘公哲 邹峰 赵永强 马文慧 亓磊[3] Wang Jizhen;Liu Gongzhe;Zou Feng;Zhao Yongqiang;Ma Wenhui;Qi Lei(Department of Thoracic and Cardiac Surgery,People’s Hospital Affiliated to Shandong First Medical University,Jinan 271199,China;Department of Spleen,Stomach,Liver and Gall,Jinan Integrated Traditional Chinese and Western Medicine Hospital,Jinan 271199,China;Department of Chest Surgery,Cheeloo College of Medicine,Shandong University,Jinan 250012,China)

机构地区：[1]山东第一医科大学附属人民医院胸心外科,济南271199 [2]济南市中西医结合医院脾胃肝胆科,济南271199 [3]山东大学齐鲁医院胸外科,济南250012

出　　处：《中华实验外科杂志》2022年第3期472-474,共3页Chinese Journal of Experimental Surgery

摘　　要：目的探讨血管生成素1(ANGPT1)在非小细胞肺癌(NSCLC)中的表达及其对肺癌细胞生物学行为的影响。方法收集40例NSCLC患者肿瘤组织及癌旁组织、NSCLC细胞系(A549、HCC827、H460和H1299)和人正常肺上皮细胞(BEAS-2B)行研究检测。转染ANGPT1真核表达质粒至A549细胞(pc-ANGPT1组),设定空载表达质粒转染空载组(pcDNA组)和空白对照组(Control组)。反转录实时荧光定量聚合酶链反应(qRT-PCR)和蛋白质印迹法(Western blot)分别检测ANGPT1基因信使核糖核酸(mRNA)和蛋白表达。细胞计数(CCK-8)检测细胞吸光度,流式细胞仪检测细胞凋亡,Transwell检测细胞迁移及侵袭。两组间比较行t检验,多组间比较采用单因素方差分析(组间比较采用SNK检验)。结果NSCLC患者肿瘤组织ANGPT1基因mRNA和蛋白表达显著低于癌旁组织(mRNA表达为0.17±0.04比1.03±0.05,蛋白表达为0.27±0.06比0.83±0.07),差异有统计学意义(t=24.145、19.381,P<0.05)。A549、HCC827、H460和H1299细胞ANGPT1基因mRNA和蛋白表达显著低于BEAS-2B细胞(mRNA表达为0.11±0.03、0.32±0.06、0.28±0.03、0.37±0.06比1.05±0.08,蛋白表达为0.23±0.02、0.29±0.05、0.27±0.04、0.35±0.05比0.79±0.06),差异有统计学意义(F=15.447、13.516,P<0.05)。pc-ANGPT1组A549细胞ANGPT1基因mRNA和蛋白表达、细胞凋亡率显著高于pcDNA和Control组[ANGPT1基因mRNA为4.24±0.13比1.01±0.05、1.02±0.04,ANGPT1基因蛋白为1.14±0.08比0.25±0.04、0.24±0.04,细胞凋亡率为(19.27±3.65)%比(3.89±0.78)%、(3.82±0.81)%],48、72、96 h细胞吸光度、细胞迁移数及侵袭数显著低于pcDNA和Control组[48 h吸光度值为0.36±0.05比0.49±0.07、0.52±0.06,72 h吸光度值为0.47±0.07比0.73±0.08、0.79±0.07,96 h吸光度值为0.61±0.08比0.97±0.11、1.03±0.09,细胞迁移数为(60.34±9.42)个比(122.73±12.59)、(121.25±14.16)个,细胞侵袭数为(29.84±5.37)个比(75.31±8.65)、(78.29±8.90)个],差异有统计学意义(F=34.166、21.542、29.942、8.929�Objective To investigate the expression of angiopoietin 1(ANGPT1)in non-small cell lung cancer(NSCLC)and its effect on the biological behavior of lung cancer cells.Methods Totally,40 patients with NSCLC were collected and NSCLC cell lines(A549,HCC827,H460 and H1299)and human bronchial epithelial cell line(BEAS-2B)were cultured.The ANGPT1 eukaryotic expression plasmid was transfected into A549 cells(pc-ANGPT1 group),and the empty-loaded expression plasmid was set to transfect the empty-loaded group(pcDNA group)and the blank control group(control group).Quantitative reverse transcription polymerase chain reaction(qRT-PCR)and Western blotting were used to detect ANGPT1 mRNA and protein expression,respectively.Cell counting kit-8(CCK-8)assay was used to detect cell absorbance,flow cytometry to detect cell apoptosis,and Transwell to detect cell migration and invasion.The t test was used for comparison between two groups,and the one-way analysis of variance was used for comparison among multiple groups(SNK test was used for comparison between groups).Results The mRNA and protein expression levels of ANGPT1 gene in tumor tissues of NSCLC patients were significantly lower than those in adjacent tissues(mRNA expression:0.17±0.04 versus 1.03±0.05,protein expression:0.27±0.06 versus 0.83±0.07),the differences were statistically significant(t=24.145,19.381,P<0.05).The mRNA and protein expression levels of ANGPT1 gene in A549,HCC827,H460 and H1299 cells were significantly lower than those in BEAS-2B cells(mRNA expression:0.11±0.03,0.32±0.06,0.28±0.03,0.37±0.06 vs.1.05±0.08,protein expression:0.23±0.02,0.29±0.05,0.27±0.04,0.35±0.05 vs.0.79±0.06),the differences were statistically significant(F=15.447,13.516,P<0.05).The mRNA and protein expression of ANGPT1 gene and the apoptosis rate of A549 cells in the pc-ANGPT1 group were significantly higher than those in the pcDNA and control groups[ANGPT1 mRNA:4.24±0.13 vs.1.01±0.05,1.02±0.04,ANGPT1 protein:1.14±0.08 vs.0.25±0.04,0.24±0.04,apoptosis rate:(19.27±3.65)

关 键 词：非小细胞肺癌 血管生成素1 生物学行为 

分 类 号：R734.2[医药卫生—肿瘤]