血清IgG N-糖链与胃癌Lauren分型的相关性  

Correlation between serum IgG N-glycan and Lauren classification of gastric cancer

作　　者：牛晓云 徐晓燕 王妍 任士芳 顾建新 Niu Xiaoyun;Xu Xiaoyan;Wang Yan;Ren Shifang;Gu Jianxin(National Health Commission Key Laboratory of Glycoconjugates Research,Department of Biochemistry and Molecular Biology,School of Basic Medical Sciences,Fudan University,Shanghai 200032,China;Department of Medical Oncology,Zhongshan Hospital,Fudan University,Shanghai 200032,China)

机构地区：[1]复旦大学基础医学院生物化学与分子生物学系国家卫健委糖复合物重点实验室,上海200032 [2]复旦大学附属中山医院肿瘤内科,上海200032

出　　处：《中华检验医学杂志》2022年第4期373-380,共8页Chinese Journal of Laboratory Medicine

摘　　要：目的分析血清IgG N-糖链与胃癌Lauren分型的相关性。方法对2017—2018年于复旦大学中山医院进行治疗的17例弥漫型和21例肠型胃癌患者进行回顾性分析。汇总并统计一般资料和临床特征。采用超高效液相色谱法定量检测血清免疫球蛋白G(IgG)N-糖链含量,比较血清IgG N-糖链在肠型与弥漫型胃癌之间的差异,并采用Logistic回归分析评估血清IgG N-糖链与Lauren分型的相关性。结果IgG N-糖链分析包括27个直接检测到的糖链和4个糖链衍生性状。H为己糖,N为乙酰葡萄糖胺,F为岩藻糖,S为唾液酸。不同化疗方案之间的IgG N-糖链差异均无统计学意义。与肠型胃癌患者相比,弥漫型胃癌患者血清中H3N3F1(t=3.785,P=0.001)、H3N4(t=3.919,P=0.002)、H3N4F1(t=2.770,P=0.005)、H3N5F1(t=2.888,P=0.010)较少;H4N4F1(6)(t=‒3.488,P<0.001)、H5N4F1(t=‒3.401,P=0.003)、H5N5F1(t=‒2.303,P=0.023)、H5N4F1S1(t=‒3.068,P=0.008)较多。H3N3F1(OR:1.20,P=0.008)、H3N4(OR:1.32,P=0.005)、H3N4F1(OR:1.13,P=0.017)、H3N5F1(OR:1.78,P=0.015)、H4N4F1(6)(OR:0.43,P=0.008)、H5N4F1(OR:0.74,P=0.008)、H5N5F1(OR:0.32,P=0.036)、H5N4F1S1(OR:0.48,P=0.009)与Lauren分型显著相关。唾液酸化(t=‒2.717,P=0.012)和半乳糖化(t=‒3.400,P=0.001)的IgG N-糖链在肠型胃癌患者血清中较少。半乳糖化(OR:0.87,P=0.007)和唾液酸化(OR:0.62,P=0.015)与Lauren分型显著相关。结论部分IgG N-糖链与胃癌Lauren分型具有显著相关性,可作为辅助Lauren分型的潜在生物标志物。Objective Analyze the correlation between serum immunoglobulin G(IgG)N-glycan and Lauren classification of gastric cancer.Methods A retrospective study was performed on 17 patients with diffuse type gastric cancer and 21 patients with intestinal type who received treatment in Zhongshan Hospital from 2017 to 2018,and the general medical history data and disease characteristics were summarized.The serum IgG glycome profiles were analyzed by ultraperformance liquid chromatography,and the difference between intestinal type and diffuse type gastric cance was compared.Logistic regression was used to evaluate the correlation between serum IgG N-glycan and Lauren classification.Results IgG N-glycome analysis included 27 directly detected glycans and 4 derived traits.H=Hexose,N=N-acetylglucosamine,F=Fucose,S=Sialic acid.There was no significant difference in IgG N-glycan among different chemotherapy protocol.Compared with intestinal type,H3N3F1(t=3.785,P=0.001),H3N4(t=3.919,P=0.002),H3N4F1(t=2.770,P=0.005),H3N5F1(t=2.888,P=0.010)were decreased in diffuse type;H4N4F1(6)(t=‒3.488,P<0.001),H5N4F1(t=‒3.401,P=0.003),H5N5F1(t=‒2.303,P=0.023),H5N4F1S1(t=‒3.068,P=0.008)were increased.H3N3F1(OR:1.20,P=0.008),H3N4(OR:1.32,P=0.005),H3N4F1(OR:1.13,P=0.017),H3N5F1(OR:1.78,P=0.015),H4N4F1(6)(OR:0.43,P=0.008),H5N4F1(6)(OR:0.74,P=0.008),H5N5F1(OR:0.32,P=0.036),H5N4F1S1(OR:0.48,P=0.009)were significantly correlated with Lauren classification.Sialylated(t=‒2.717,P=0.012)and galactosylated(t=‒3.400,P=0.001)IgG N-glycan were reduced in patients with intestinal type gastric cancer.Galactosylated(OR:0.87,P=0.007)and sialylated(OR:0.62,P=0.015)IgG N-glycan were significantly correlated with Lauren classification.Conclusion Some IgG N-glycan are significantly correlated with Lauren classification,which can be used as potential biomarkers.

关 键 词：胃肿瘤 色谱法 液相 N-糖基化 Lauren分型 

分 类 号：R735.2[医药卫生—肿瘤]