Ultra-performance Liquid Chromatography-Quadrupole/Time-of-Flight Mass Spectrometry Based Bile and Urine Metabonomics Study on the Ameliorative Effects of Curcuma wenyujin Rhizoma on Acute Blood Stasis in Rats  

作　　者：Min Hao Meng-Ting Zhao Huang-Jin Tong De Ji Lin Li Lian-Lin Su Wei Gu Chun-Qin Mao Tu-Lin Lu 

机构地区：[1]Chinese Medicine Processing Engineering Center,School of Pharmaceutical Sciences,Zhejiang Chinese Medical University,Hangzhou,China [2]Chinese Medicine Processing Laboratory,School of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guizhou,China [3]Department of Pharmacy,Affiliated Hospital of Integrated Traditional Chinese and Western Medicine,Nanjing University of Chinese Medicine,Nanjing,China [4]Chinese Medicine Processing Laboratory,College of Pharmacy,Nanjing University of Chinese Medicine,Nanjing,China

出　　处：《World Journal of Traditional Chinese Medicine》2022年第1期141-152,共12页世界中医药杂志（英文）

基　　金：financially supported by the National Natural Science Foundation of China (81673598, 81973483, 82003948);Natural Science Foundation of Zhejiang Province (LQ21H280002);The National Key Research and Development Program of China (2018YFC1707000);the National traditional Chinese medicine industry special project (2015468002-2);National Standardization Program for Chinese Medicine (ZYBZH-Y-SC-40);Zhejiang Chinese Medicine University School-level Scientific Research Fund for Talents (2020ZR14)。

摘　　要：Background: Curcuma wenyujin rhizome(CWR) is a commonly used Chinese herbal medicine for treating blood stasis in China for 1000 of years. However, the underlying mechanism of CWR remains unclear. Aims and Objectives: The purpose of this study is to clarify the bioactive mechanism of CWR in treating blood stasis. Materials and Methods: In this study, pharmacological indexes, including hemorheology and four blood coagulation indexes were tested. Bile and urine metabolomics were engaged by UPLC-Q/TOF-MS. Multivariate statistical analysis were used to screen out differential endogenous metabolites. Results: The results indicated that CWR significantly ameliorated the hemorheology and coagulation functions of acute blood stasis(ABS) model rats. Moreover, 27 endogenous metabolites between the CWR group and the ABS group were screened, and the levels were all improved to certain degrees by CWR preadministration. Metabonomics results indicated that ABS was mainly related to linoleic acid metabolism, arachidonic acid metabolism, glycerophospholipid metabolism, sphingolipid metabolism, pentose and glucuronate intercereasonversions, steroid hormone biosynthesis, and primary bile acid biosynthesis. Conclusion: In a word, the metabolomics method is consistent with the holistic view of traditional Chinese medicine(TCM) that can be a powerful means to illustrate the biological activity mechanism of CWR in treating blood stasis and to offer research demonstration for further study on the effector mechanism of TCM.

关 键 词：BILE Curcuma wenyujin rhizome metabolomics ultra-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry URINE 

分 类 号：R285.5[医药卫生—中药学]