MCM2、MCM4、MCM10在胰腺癌中的表达和预后价值及其作用机制  

Expression and Prognostic Value of MCM2,MCM4,MCM10 in Pancreatic Cancer and Its Action Mechanism

作　　者：贾如江[1] 赵树山 尹清臣[1] 刘秀丽 杨龙龙[1] 温桂海[1] JIA Rujiang;ZHAO Shushan;YIN Qingchen;LIU Xiuli;YANG Longlong;WEN Guihai(The First Department of General Surgery,Handan Central Hospital,Handan 056102,China)

机构地区：[1]邯郸市中心医院普一科,邯郸056102

出　　处：《激光生物学报》2022年第2期173-179,共7页Acta Laser Biology Sinica

基　　金：河北省卫健委重点科技研究计划项目(20200449)。

摘　　要：基于生物信息学分析MCM2、MCM4、MCM10在胰腺癌中的表达及其与患者预后的关系,胰腺癌组织MCM2、MCM4、MCM10的表达明显高于正常胰腺组织(P<0.05),MCM2、MCM4、MCM10 mRNA表达水平与胰腺癌患者的无病生存率(DFS)、总生存率(OS)显著相关(P<0.05)。高表达MCM2、MCM4、MCM10的胰腺癌患者总生存期较差(P<0.05)。进一步探讨MCM2、MCM4、MCM10参与胰腺癌发生发展的机制,发现MCM2、MCM4、MCM10表达与胰腺癌的发生具有明显的相关性,与其具有相互作用的蛋白有MCM5、MCM6、RPA3、RPA1、POLA2、ORC2、ORC3、DBF4、CDC6、GINS2等。MCM2、MCM4、MCM10主要富集在前复制复合体的活化、DNA复制、DNA合成、DNA复制的启动、细胞核DNA的复制、CDC6相关的复制起点识别复合物、皮瓣转移、介导通路、体温自动调节通路等信号通路中。MCM2、MCM4、MCM10在胰腺癌组织中呈现高表达,与患者预后显著相关。MCM2、MCM4、MCM10表达具有明显相关性,其构成功能模块,通过前复制复合体的活化、DNA复制、DNA合成、DNA复制的启动、细胞核DNA的复制、CDC6相关的复制起点识别复合物等生物过程参与胰腺癌细胞的发生与发展。MCM2、MCM4、MCM10在胰腺癌中功能模块的发现有望为胰腺癌的靶标治疗提供新的方向。The expressions of MCM2,MCM4 and MCM10 in pancreatic cancer and their relationship with prognosis were analyzed based on bioinformatics.The expressions of MCM2,MCM4 and MCM10 in pancreatic cancer tissues were significantly higher than those in normal pancreatic tissues(P<0.05).Expression levels of MCM2,MCM4 and MCM10 mRNA were significantly associated with disease-free survival(DFS)and overall survival(OS)in pancreatic cancer patients(P<0.05).There was significant correlation between DFS and OS in cancer patients(P<0.05).Patients with high expression of MCM2,MCM4 and MCM10 had poorer overall survival(P<0.05).We further studied the mechanism and found that the expressions of MCM2,MCM4,and MCM10 were significantly correlated with certain proteins,and the interacting proteins were MCM5,MCM6,RPA3,RPA1,POLA2,ORC2,ORC3,DBF4,CDC6,GINS2,etc.MCM2,MCM4 and MCM10 are mainly enriched in signaling pathways such as preactivation replication complex,DNA replication,DNA synthesis,DNA replication initiation,nuclear DNA replication,CDC6-associated replication start recognition complex,skin flap transfer,mediated pathways,and body temperature auto-regulation pathways.The high expression of MCM2,MCM4 and MCM10 in pancreatic cancer tissues were significantly correlated with the prognosis of patients.The expression of MCM2,MCM4 and MCM10 have obvious correlation with certain factors,which constitute a functional module,participating in the occurrence and development of pancreatic cancer cells through preactivation replication complex,DNA replication,DNA synthesis,DNA replication initiation,nuclear DNA replication,CDC6-associated replication start recognition complex.The discovery of functional modules of MCM2,MCM4 and MCM10 in pancreatic cancer is expected to provide a new prospect for the targeted therapy of pancreatic cancer.

关 键 词：胰腺癌 MCM2 MCM4 MCM10 预后分析 

分 类 号：R735.9[医药卫生—肿瘤]