山药多糖的氨取代修饰和保润性能研究  

Study on ammonia substitution modification and moisture retention properties of yam polysaccharides

作　　者：白家峰[1] 熊亚妹 刘远上 孙懿岩 刘绍华[1] 贾学伟 许春平 BAI Jia-feng;XIONG Ya-mei;LIU Yuan-shang;SUN Yi-yan;LIU Shao-hua;JIA Xue-wei;XU Chun-ping(Technical Center of China Tobacco Guangxi Industrial Co.,Ltd.,Nanning 530001,China;College of Food and Biology Engineering,Zhengzhou University of Light Industry,Zhengzhou 450002,China;China Tobacco Hebei Industrial Co.,Ltd.,Shijiazhuang 050000,China)

机构地区：[1]广西中烟工业有限责任公司技术中心,南宁530001 [2]郑州轻工业大学食品与生物工程学院,郑州450002 [3]河北中烟工业有限责任公司,石家庄050000

出　　处：《湖北农业科学》2022年第8期115-119,168,共6页Hubei Agricultural Sciences

基　　金：国家自然科学基金联合项目(U1604176);河南省科技创新团队计划(20IRTSTHN022)。

摘　　要：通过碱提法提取山药多糖,对其进行氨取代修饰,采用GC-MS、红外吸收光谱、光散射和核磁共振波谱分析其化学结构和分子构象,并对山药多糖、氨取代山药多糖与丙二醇的保润性能进行了对比。结果表明,①山药多糖是一种杂多糖,主要由葡萄糖(86.1%)、甘露糖(9.3%)、半乳糖(7.2%)组成。糖链残基组成主要有→4)-Glcp-(1→、→6)-Glcp-(1→、→6)-Galp-(1→、→3)-Manp-(1→、→2,3)-Manp-(1→和端基Glcp-(1→。②通过一维核磁分析出山药多糖为α-1,4-葡聚糖。③山药多糖的摩尔质量为1.747×10^(5)g/mol,远大于氨取代山药多糖的摩尔质量1.115×10^(4)g/mol,另外经过氨基化修饰后,多糖的多分散系数增加,摩尔质量分布变宽。④山药多糖和氨取代山药多糖都有一定的保润效果,且氨取代山药多糖的保润性能更好。The yam polysaccharide was extracted by alkaline extraction and then subjected to ammonia substitution modification.The chemical structure and molecular conformations were analyzed by GC-MS analysis,fourier transform infrared spectroscopy,light scat⁃tering analysis and nuclear magnetic resonance spectroscopy.And the moisture retention properties of yam polysaccharides,ammoniasubstituted yam polysaccharides and propylene glycol were compared.The results showed that yam polysaccharide was a heteropolysac⁃charide,mainly composed of glucose(86.1%),mannose(9.3%),galactose(7.2%).The chemical chain was mainly composed of→4)-Glcp-(1→、→6)-Glcp-(1→、→6)-Galp-(1→、→3)-Manp-(1→,→2,3)-Manp-(1→and terminal Glcp-(1→.The yam polysaccharide could be analyzed asα-1,4-glucan by 1-D NMR.The molecular weight of the yam polysaccharide was 1.747×10^(5)g/mol,which was much larger than the molecular weight of ammonia-substituted yam polysaccharide 1.115×10^(4)g/mol.In addition,after the aminogenization modification,the polydispersion coefficient of the polysaccharide increased and the molecular weight distribution wid⁃ened.The yam polysaccharide and ammonia-substituted yam polysaccharide had a certain moisturizing effect,and the moisturizing performance of ammonia-substituted yam polysaccharide was better.

关 键 词：山药多糖 氨取代 保润性能 结构分析 

分 类 号：TS426[农业科学—烟草工业]