微电解无菌水系统在实验动物屏障设施中应用的跟踪监测  

Tracking and Monitoring on Application of Micro-electrolyzed Sterile Water System in Laboratory Animal Barrier Facilities

作　　者：张薇[1] 唐彬[1] 赵勇[1] 张志妮 邓新燕[1] ZHANG Wei;TANG Bin;ZHAO Yong;ZHANG Zhini;DENG Xinyan(Laboratory Animal Center of Sun Yat-sen University,Guangzhou 510006,China)

机构地区：[1]中山大学实验动物中心,广州510006

出　　处：《实验动物与比较医学》2022年第2期117-121,共5页Laboratory Animal and Comparative Medicine

基　　金：广东省科技基础条件建设项目:广东省实验动物大小鼠资源公共服务平台建设(2013B060300010)。

摘　　要：目的跟踪观察实验动物屏障设施长期应用微电解无菌水系统是否可以持续维持终端供水的无菌。方法在2018年4月—2022年3月期间,每季度跟踪监测中山大学实验动物中心生产繁殖SPF级大小鼠的屏障设施中微电解无菌水系统1次。设置8个采样点,包括微电解出水口进入输水管道前取样点1个、终端出水口6个、输水管网回水取样点1个。按无菌操作流程取样,依据国家标准GB 14925—2010、GB/T 14926.41—2001进行微电解无菌水微生物检测。结果2018年4月—2022年3月共进行17次采样;在微电解无菌水系统正常运行期间,8个采样点的微电解水均检测无菌,符合国家标准。结论微电解无菌水系统能持续维持终端供水的无菌,可以长期应用于实验动物屏障设施。Objective To track and observe whether the long-term application of micro-electrolyzed sterile water system in laboratory animal barrier facilities can continuously maintain the terminal water aseptic.Methods From April 2018 to March 2022,the microbiological detection of the micro-electrolyzed sterile water system had been tracked and monitored once each quarter in the barrier facilities of producing and breeding SPF grade rats and mice in Laboratory Animal Center of Sun Yat-sen University.Totally 8 sampling points were set,including one sampling point before the intake of the microelectrolytized water outlet into the water duct,six sampling points at the terminal water outlet,and one sampling point for the water return of the water network.Samples were taken according to aseptic operation procedures,and microelectrolysis aseptic water was detected according to the national standards GB 14925—2010 and GB/T 14926.41—2001.Results From April 2018 to March 2022,a total of 17 tests were conducted.During the normal operation of the micro-electrolyzed sterile water system,the quality of micro-electrolyzed water at 8 sampling points was sterile,in line with the national standard.Conclusion Micro-electrolyzed sterile water system can maintain the sterility of the terminal water supply,and can be used in laboratory animal barrier facilities for a long time.

关 键 词：微电解 无菌水 监测 屏障设施 实验动物 

分 类 号：Q95-33[生物学—动物学] R-332[医药卫生]