基于叶绿体基因组重建中国野生葡萄的系统发育关系  

Recovering of Chinese wild grapes (Vitaceae) phylogeny based on chloroplast genomes

作　　者：张丽娜[1] 何华勤[1] 田甜[1] 王亚男 吴题 郑英杰 ZHANG Lina;HE Huaqin;TIAN Tian;WANG Yanan;WU Ti;ZHENG Yingjie(College of Life Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)

机构地区：[1]福建农林大学生命科学学院,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2022年第3期339-350,共12页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：福建省科技计划项目(KJD19034A).

摘　　要：野生葡萄类群有一半以上分布在中国.学者们基于形态特征和核基因组对其系统发育关系进行了研究,但结果存在比较大的冲突,类群间的亲缘关系仍未明确.为探究基于叶绿体基因组重建的中国野生葡萄的系统发育关系与前人基于形态特征或核基因组的研究结果是否一致,本研究从已公布的重测序数据中组装出26个中国野生葡萄的叶绿体基因组,并使用联合分析和溯祖理论重建了这26个类群的系统发育关系.联合分析的结果将中国野生葡萄分成了3个分支,这与前人的研究结果也存在较大冲突;而基于溯祖理论的分析没有得到较高分辨率的系统发育关系,这可能是由于单个基因的系统发育信息比较低,限制了该理论的应用.另外,相对于编码区和非编码区数据,叶绿体全基因组数据对中国野生葡萄系统发育关系的解析能力较好.About half of world wild grape genera are distributed in China, while the phylogenetic relationships based on morphological characteristics and nuclear genomes were conflicting, indicating the ambiguity in the relationships of Chinese wild grapes. To study whether the phylogenetic relationship based on chloroplast genomes is consistent with the results based on nuclear genomes or morphology, 26 chloroplast genomes of Chinese wild grapes were assembled from published resequencing datasets, then the phylogeny of the 26 species was inferred by concatenated method and coalescent method. The results showed that Chinese wild grapes were classfied into 3 lineages by concatenated analysis, which was not consistent with the classifications based on morphological features nor nuclear genomes. Coalescent method was unable to reconstruct the phylogenetic relationship of Chinese wild grapes, which may be due to the lack of phylogenetic information on individual gene. In general, whole chloroplast genome outperformed the sequences of coding and noncoding regions in interpreting the phylogeny of Chinese wild grapes.

关 键 词：叶绿体基因组 不一致性 系统发育 葡萄属 

分 类 号：Q941[生物学—植物学]