长链非编码RNA AC138128.1靶向调控microRNA-9对胃癌细胞增殖及凋亡的影响  

Effects of lncRNA AC138128.1 targeting microRNA-9 on the proliferation and apoptosis of gastric cancer cells

作　　者：陈涛[1] 刘鹏军[1] 徐敏 王彦舟 承优裕 CHEN Tao;LIU Pengjun;XU Min;WANG Yanzhou;CHENG Youyu(Department of Gastroenterology,Wujin Hospital Affiliated to Jiangsu University,Wujin Clinical College of Xuzhou Medical University,Changzhou,Jiangsu 213000,China)

机构地区：[1]江苏大学附属武进医院消化内科,徐州医科大学武进临床学院,江苏常州213000

出　　处：《徐州医科大学学报》2022年第4期268-272,共5页Journal of Xuzhou Medical University

基　　金：常州市武进区卫健局面社科发展项目(WS201920)。

摘　　要：目的研究长链非编码RNA AC138128.1(lncRNA AC138128.1)对胃癌细胞增殖和凋亡的影响及microRNA-9(miR-9)在其中的作用。方法采用质粒转染的方式构建过表达lncRNA AC138128.1和(或)过表达miR-9的胃癌细胞系,采用qRT-PCR验证两者表达水平,采用CCK8检测细胞增殖能力,采用TUNEL法检测细胞凋亡水平,通过裸鼠实验评价胃癌细胞的在体成瘤能力。结果lncRNA AC138128.1在胃癌细胞系中低表达;上调lncRNA AC138128.1可显著抑制胃癌细胞系增殖、促进凋亡。在体实验中,上调lncRNA AC138128.1可有效降低胃癌细胞的成瘤能力。上调miR-9可显著逆转lncRNA AC138128.1对胃癌细胞增殖和凋亡的影响。结论lncRNA AC138128.1可能通过调控miR-9发挥抑制胃癌细胞增殖及促进凋亡的作用,提示lncRNA AC138128.1可能对胃癌治疗具有正向积极作用。Objective To investigate the effects of long-chain noncoding RNA AC138128.1(lncRNA AC138128.1) on the proliferation and apoptosis of gastric cancer cells and the role of microRNA-9(miR-9).Methods Gastric cancer cells overexpressing lncRNA AC138128.1 and/or miR-9 were constructed through plasmid transfection.The levels of lncRNA AC138128.1 and miR-9 were verified by qRT-PCR.The cellular proliferation ability was detected by CCK8 assay,and the apoptosis was detected by TUNEL assay.The tumorigenicity of gastric cancer cells in vivo was evaluated by nude mouse experiment.Results LncRNA AC138128.1 was lowly expressed in gastric cancer cells.Up-regulation of lncRNA AC138128.1 significantly inhibited the proliferation of gastric cancer cells but promoted their apoptosis.In vivo up-regulation of lncRNA AC138128.1 effectively reduced the tumorigenicity of gastric cancer cells.Up-regulation of miR-9 significantly reversed the effects of lncRNA AC138128.1 on the proliferation and apoptosis of gastric cancer cells.Conclusions LncRNA AC138128.1 may inhibit the proliferation and promote apoptosis of gastric cancer cells by regulating miR-9,suggesting that lncRNA AC138128.1 may play a positive role in the treatment of gastric cancer.

关 键 词：长链非编码RNA AC138128.1 microRNA-9 胃癌 增殖 凋亡 

分 类 号：R735.2[医药卫生—肿瘤]