胡椒碱调控STAT3信号通路抑制胃癌的机制研究  

Study on the mechanism of piperine regulating the STAT3 signaling pathway to inhibit gastric cancer

作　　者：陈镝 陈美玲 刘瀚予 姜怡 白芙婷 郭宇航 朱隆基 武晓慧[2] Rajiv Kumar Jha CHEN Di;CHEN Mei-ling;LIU Han-yu;JIANG Yi;BAI Fu-ting;GUO Yu-hang;ZHU Long-ji;WU Xiao-hui;Rajiv Kumar Jha(Department of Basic Medical Science, Xi′an Medical University, Xi′an 710021, China;College of Clinical Medicine, Xi′an Medical University, Xi′an 710021, China)

机构地区：[1]西安医学院基础医学部,陕西西安710021 [2]西安医学院临床医学院,陕西西安710021

出　　处：《陕西科技大学学报》2022年第3期72-76,99,共6页Journal of Shaanxi University of Science & Technology

基　　金：陕西省教育厅专项科研计划项目(21JK0893);西安医学院中尼友好拉吉姆医学实验室开放基金项目(18LJM07);西安医学院博士科研启动基金项目(2020DOC04,2021DOC10);西安医学院大学生创新创业训练计划项目(202111840010)。

摘　　要：胡椒碱可通过不同信号途径诱导胃癌细胞凋亡,而其对STAT3信号通路的调控作用仍有待阐明.用MTT实验检测胡椒碱抑制胃癌细胞增殖的作用,用Annexin V-FITC/PI双染实验检测胡椒碱诱导胃癌细胞凋亡的作用,用裸鼠荷瘤实验检测胡椒碱在体内抑制肿瘤生长的作用,通过蛋白免疫印迹实验检测胡椒碱调控STAT3信号通路的分子机制.结果显示,胡椒碱既可在体外诱导胃癌细胞凋亡从而抑制其增殖,也可在裸鼠体内抑制肿瘤生长.其机制是胡椒碱通过抑制STAT3活化并降低Survivin表达,从而减弱胃癌细胞逃避凋亡的能力,实现抑制胃癌的作用.胡椒碱对STAT3信号通路的调控作用表明其有望成为新的胃癌辅助药物.Piperine induce apoptosis of gastric cancer cells through different pathways,while its regulatory effect on STAT3 signaling pathway remains clarified.The MTT assay was performed to detect the effect of piperine on inhibiting the proliferation of gastric cancer cells.The Annexin V-FITC/PI double staining assay was performed to detect the effect of piperine on apoptosis of gastric cancer cells.And the effect of piperine on inhibiting tumor growth in vivo was detected by the nude mouse tumor-bearing experiment.The molecular mechanism of piperine regulating the STAT3 signaling pathway was detected by western blot assay.The results showed that piperine could not only induce apoptosis to inhibit gastric cancer cells proliferation,but also inhibit tumor growth in nude mice.The underlying mechanism was that piperine inhibited the activation of STAT3 and reduced the expression of Survivin,thereby attenuating the ability of gastric cancer cells to escape apoptosis and so inhibiting gastric cancer.The regulatory effect of piperine on STAT3 signaling pathway indicated that it was expected to become a new adjuvant drug for gastric cancer therapy.

关 键 词：胡椒碱 STAT3 胃癌 凋亡 

分 类 号：R735.2[医药卫生—肿瘤]