结直肠癌组织的长链非编码RNA CAR10水平及临床意义  

Level and clinical significance of long chain non-coding RNA CAR10 in colorectal cancer tissues

作　　者：张兰芳[1] 秦蕾[1] 薛耀峰 董戴源 李光艳[1] ZHANG Lanfang;QIN Lei;XUE Yaofeng;DONG Daiyuan;LI Guangyan(Department of Gastroenterology, the First Affiliated Hospital of Xinxiang Medical College, Weihui 453100, China)

机构地区：[1]新乡医学院第一附属医院消化内科,河南卫辉453100

出　　处：《临床肿瘤学杂志》2022年第4期367-371,共5页Chinese Clinical Oncology

摘　　要：目的探讨结直肠癌组织的长链非编码RNA染色质相关RNA 10(CAR10)水平及临床意义。方法采用实时定量PCR(qPCR)检测91对结直肠癌组织和癌旁组织的CAR10水平,分析组织CAR10水平与结直肠癌常见临床病理特征的关系,受试者工作特征(ROC)曲线分析CAR10水平诊断结直肠癌的效能,采用Kaplan-Meier法分析CAR10水平与预后的关系,应用Cox比例风险模型对影响结直肠癌预后的独立因素进行多因素分析。结果结直肠癌组织的CAR10水平为5.493±1.745,高于对应癌旁组织的2.371±1.046(P<0.05)。ROC曲线分析显示组织CAR10水平诊断结直肠癌的曲线下面积为0.925(95%CI:0.889~0.961),当最佳截断值为4.268时约登指数最大,灵敏度和特异度分别为74.73%和96.70%。组织CAR10水平与性别、年龄、肿瘤大小和肿瘤部位无关(P>0.05),而与淋巴结转移、远处转移、浸润深度、分化程度和TNM分期有关(P<0.05)。单因素分析淋巴结转移、分化程度、浸润深度、TNM分期和CAR10水平与结直肠癌患者的总生存期(OS)有关,其中CAR10低水平组的中位OS为60.0个月,优于高水平组的35.0个月(HR=5.068,95%CI:3.238~7.124,P=0.004),进一步多因素分析发现组织CAR10水平是预后不良的危险因素(HR=4.456,95%CI:3.514~5.987,P=0.016)。结论结直肠癌组织CAR10水平升高且与肿瘤的恶性程度和不良预后有关,可作为结直肠癌预后的潜在分子标志物。Objective To investigate the level and clinical significance of long chain non-coding RNA chromatin-associated RNA 10(CAR10)in colorectal cancer tissues.Methods Real-time quantitative PCR(qPCR)was used to detect the CAR10 level in 91 pairs of colorectal cancer tissues and adjacent tissues.Relationship between the CAR10 level and the common clinicopathological features of colorectal cancer was analyzed.Efficacy of CAR10 level in the diagnosis of colorectal cancer was evaluated by receiver operating characteristic(ROC)curve.Kaplan-Meier method was used to analyze the relationship between CAR10 level and prognosis.Cox proportional hazards model was used to analyze the independent factors affecting the prognosis of colorectal cancer.Results The CAR10 level in colorectal cancer tissues was 5.493±1.745,which was higher than 2.371±1.046 in adjacent tissues(P<0.05).ROC curve analysis showed that the area under the curve of tissue CAR10 level in the diagnosis of colorectal cancer was 0.925(95%CI:0.889 to 0.961).When the optimal cut-off value was 4.268,the Yoden index was the largest with sensitivity of 74.73%and specificity of 96.70%.CAR10 level was not related to gender,age,tumor size and tumor location(P>0.05),but related to lymph node metastasis,distant metastasis,depth of invasion,degree of differentiation and TNM stage(P<0.05).Univariate analysis showed that lymph node metastasis,degree of differentiation,depth of invasion,TNM stage and CAR10 level were related to overall survival(OS)in patients with colorectal cancer.The median OS in the low-level CAR10 group was 60.0 months,which was better than 35.0 months in the high-level CAR10 group(HR=5.068,95%CI:3.238 to 7.124,P=0.004).Further multivariate analysis found that the level of tissue CAR10 was a risk factor for poor prognosis(HR=4.456,95%CI:3.514 to 5.987,P=0.016).Conclusion The increased CAR10 level in colorectal cancer is related to the degree of malignancy and poor prognosis,which can be used as a potential molecular marker for the prognosis of colorectal can

关 键 词：结直肠癌 长链非编码RNA 染色质相关RNA 10 临床意义 预后 

分 类 号：R735.3[医药卫生—肿瘤]