IL-10、TGF-β1及iNOS在不同病理分型声带白斑中的表达及意义  

Expression and Clinical Significance of Interleukin-10,Transforming Growth Factor-β1 and Inducible Nitric Oxide Synthase in the Process of Vocal Fold Leukoplakia Canceration

作　　者：游永晶 马艳利[2] 刘婷[3] 庄佩耘 You Yongjing;Ma Yanli;Liu Ting;Zhuang Peiyun(The School of Clinical Medicine,Fujian Medical University,Fuzhou,350108,China;不详)

机构地区：[1]福建医科大学临床医学部,福州350108 [2]厦门大学附属中山医院 [3]厦门大学分子影像暨转化医学研究中心

出　　处：《听力学及言语疾病杂志》2022年第3期257-261,共5页Journal of Audiology and Speech Pathology

基　　金：福建省自然科学基金(2020J011212)资助。

摘　　要：目的评估白介素-10(IL-10)、转化生长因子-β1(TGF-β1)及诱导型一氧化氮合酶(iNOS)在不同病理分级声带白斑中的表达,探讨其与声带白斑癌变的关系。方法通过免疫组织化学染色技术和计算机图像定量分析法,检测IL-10、TGF-β1及iNOS在40例临床诊断为声带白斑且病理分型分别为10例鳞状上皮单纯性增生、10例鳞状上皮轻度不典型增生、10例鳞状上皮中重度不典型增生、10例声门型喉癌及10例声带息肉中的表达,比较各组结果。结果随着声带上皮细胞异型程度的增加,IL-10表达量逐渐升高,在声带鳞状上皮单纯性增生、轻度不典型增生、中重度不典型增生与声门型喉癌间比较差异均有统计学意义(P<0.05),其表达在声带鳞状上皮单纯性增生和声带息肉间比较差异无统计学意义(P>0.05)。iNOS和TGF-β1的表达除在声带鳞状上皮单纯性增生和声带息肉间比较差异无统计学意义(P>0.05)外,其他各组间的表达比较差异均有统计学意义(P<0.05),两者的表达量在声带鳞状上皮单纯性增生、轻度不典型增生、中重度不典型增生中呈递增趋势,而中重度不典型增生进展为喉癌时,表达量降低。结论TGF-β1和iNOS的表达量改变可能为声带白斑癌变过程中的早期事件,IL-10可作为声带白斑早期癌变的分子标记物之一。Objective To assess the expression of interleukin-10(IL-10),transforming growth factor-β1(TGF-β1)and inducible nitric oxide synthase(iNOS)in different pathological grades of vocal fold leukoplakia,and to explore their roles in the process of vocal fold leukoplakia canceration.Methods Ten cases of vocal fold leukoplakia with simple hyperplasia of squamous epithelium,ten of mild epithelial dysplasia,ten of moderate to severe epithelial dysplasia,ten of vocal fold leukoplakia canceration and ten of vocal polyp were enrolled in the study.Immunohistochemical analysis and quantitative image analysis were used to detect the expression level of interleukin-10,transforming growth factorβ-1 and inducible nitric oxide synthase of the selected pathological samples.Results With the increase of dysplasia degree of the epithelial cells of vocal fold,the interleukin-10 was increasingly expressed.There was statistical significance among the vocal fold leukoplakia with simple hyperplasia of squamous epithelium,mild epithelial dysplasia,moderate to severe epithelial dysplasia and vocal fold leukoplakia canceration(P<0.05).The comparison between the vocal fold leukoplakia with simple hyperplasia of squamous epithelium and vocal polyp was not significanty differente(P>0.05).The expression level of both transforming growth factorβ-1 and inducible nitric oxide synthase was statistically significancte among the above groups(P<0.05),except the comparison between the vocal fold leukoplakia with simple hyperplasia of squamous epithelium and vocal polyp(P>0.05).The positive expression rate of both transforming growth factorβ-1 and inducible nitric oxide synthase showed an increasing trend from vocal fold leukoplakia with simple hyperplasia of squamous epithelium,mild epithelial dysplasia to moderate and severe epithelial dysplasia,but a reduction trend when vocal fold leukoplakia with moderate to severe dysplasia worsened to laryngeal carcinoma.Conclusion Transforming growth factorβ-1 and inducible nitric oxide synthase may be the ear

关 键 词：声带白斑 白介素-10 转化生长因子-Β1 诱导型一氧化氮合酶 

分 类 号：R767.4[医药卫生—耳鼻咽喉科]