脆弱拟杆菌BF839可改善Fmr1 KO小鼠的学习记忆能力及社交新奇偏好能力  

Bacteroides fragilis BF839 can improve learning,memory and social novelty of fragile X-mental retardation gene 1 knockout mice

作　　者：林楚慧[1] 曾婷[1] 林键泓 肖枫 段现来 陈盛强[2] 邓宇虹[1] Lin Chuhui;Zeng Ting;Lin Jianhong;Xiao Feng;Duan Xianlai;Chen Shengqiang;Deng Yuhong(Department of Clinical Nutrition,Second Affiliated Hospital of Guangzhou Medical University,Guangzhou 510260,China;Medical Administration College,Guangzhou Medical University,Guangzhou 510182,China;Department of Neurology,Third Hospital of Changsha,Changsha 410015,China)

机构地区：[1]广州医科大学附属第二医院临床营养科,广州510260 [2]广州医科大学卫生管理学院,广州511483 [3]长沙市第三医院神经内科,长沙410015

出　　处：《中华神经医学杂志》2022年第4期341-347,共7页Chinese Journal of Neuromedicine

基　　金：广州市卫生健康科技项目一般引导项目(20201A011081);湖南省卫生健康委科研计划课题项目(A20182006)。

摘　　要：目的观察脆弱拟杆菌BF839干预对脆性X智力低下基因1(Fmr1)敲除(Fmr1 KO)小鼠学习记忆能力及社交新奇偏好能力的影响。方法将3周龄30只FVB系Fmr1 KO小鼠按随机数字表法分为Fmr1 KO组(n=15)和Fmr1 KO+BF839组(n=15),Fmr1 KO组小鼠每天自由饮用高压灭菌自来水,Fmr1 KO+BF839组小鼠每天饮用BF839菌液(10 mL/d)。另取11只每天自由饮用高压灭菌自来水的野生型(WT)小鼠作为对照(WT组)。干预4周后,采用Morris水迷宫实验观察各组小鼠间逃避潜伏期、穿越原平台次数的差异,采用三箱社交实验观察各组小鼠间与陌生小鼠接触次数、接触时间的差异。结果Fmr1 KO组小鼠第4天的逃避潜伏期[(46.06±10.29)s]明显高于WT组[(33.39±12.02)s],Fmr1 KO+BF839组小鼠第4天的逃避潜伏期[(28.39±9.07)s]明显低于Fmr1 KO组,差异均有统计学意义(P<0.05);Fmr1 KO+BF839组小鼠第4天的逃避潜伏期略低于WT组,差异无统计学意义(P>0.05)。Fmr1 KO组小鼠穿越原平台次数[0.00(0.00,1.00)次]略低于WT组[1.00(0.00,1.00)次],差异无统计学意义(P>0.05);Fmr1 KO+BF839组小鼠穿越原平台次数[1.50(1.00,2.00)次]明显高于Fmr1 KO组及WT组,差异均有统计学意义(P<0.05)。Fmr1 KO组小鼠与陌生小鼠的接触次数[5.50(0.50,12.75)次]少于WT组[7.00(4.00,17.00)次],但差异无统计学意义(P>0.05);Fmr1 KO+BF839组小鼠与陌生小鼠的接触次数[23.00(16.00,36.00)次]明显多于Fmr1 KO组及WT组,差异均有统计学意义(P<0.05)。Fmr1 KO组小鼠与陌生小鼠的接触时间[9.50(0.50,41.95)s]明显少于WT组[142.00(65.00,171.60)s],差异有统计学意义(P<0.05);Fmr1 KO+BF839组小鼠与陌生小鼠的接触时间[69.60(50.40,98.40)s]明显多于Fmr1 KO组,差异有统计学意义(P<0.05);Fmr1 KO+BF839组小鼠与陌生小鼠的接触时间少于WT组,但差异无统计学意义(P>0.05)。结论BF839早期干预能明显提高Fmr1 KO小鼠的学习记忆能力及社交新奇偏好能力,甚至使Fmr1 KO小鼠恢复至正常水平,�Objective To observe the effect of bacteroides fragilis BF839 intervention on learning,memory and social novelty of fragile X-mental retardation gene 1(Fmr1)knockout(KO)mice.Methods Thirty three-week-old Fmr1 KO mice were randomly divided into Fmr1 KO group(n=15)and Fmr1 KO+BF839 group(n=15).Mice in the Fmr1 KO group freely drank autoclaved tap water everyday;mice in the Fmr1 KO+BF839 group drank BF839 bacterial liquid(10 mL/d)everyday;11 wild-type mice freely drank autoclaved tap water everyday were set as controls(WT group).After 4 weeks of intervention,Morris water maze test was used to observe the differences in escape latency and frequencies of crossing the original platform among mice in each group;Three-chamber Social Interaction Test was used to observe the differences in contact frequencies and contact durations with unfamiliar mice among mice in each group.Results On the 4th d of experiment,the escape latency of mice in the Fmr1 KO group([46.06±10.29]s)was significantly longer than that in the WT group([33.39±12.02]s,P<0.05);the escape latency of mice in the Fmr1 KO+BF839 group([28.39±9.07]s)was significantly shorter than that in the Fmr1 KO group(P<0.05);the escape latency of mice in the Fmr1 KO+BF839 group was slightly shorter than that in the WT group without significant difference(P>0.05).The frequencies of crossing through the original platform of mice in Fmr1 KO group(0.00[0.00,1.00]time)was slightly less than that in WT group(1.00[0.00,1.00]time)without significant difference(P>0.05);that in the Fmr1 KO+BF839 group(1.50[1.00,2.00]times)was significantly larger than that in the Fmr1 KO group and WT group(P<0.05).The contact frequencies of the mice in the Fmr1 KO group with unfamiliar mice(5.50[0.50,12.75]times)was less than that in the WT group(7.00[4.00,17.00]times)without significant difference(P>0.05);that in the Fmr1 KO+BF839 group(23.00[16.00,36.00]times)was significantly increased as compared with that in the Fmr1 KO group and WT group(P<0.05).The contact duration of mice in the Fmr1 KO g

关 键 词：脆弱拟杆菌839 脆性X综合征 孤独症 学习记忆能力 社交新奇偏好能力 

分 类 号：R749.94[医药卫生—神经病学与精神病学]