多指标综合评分法优化金蕾颗粒水提醇沉工艺  

Optimization of Water Extraction and Alcohol Precipitation Process of Jinlei Granules by Multi-index Comprehensive Scoring Method

作　　者：曲馨 薄双琴 柳娜 寇亮 陈晖 景明 QU Xin;BO Shuangqin;LIU Na;KOU Liang;CHEN Hui;JING Ming(College of Pharmacy,Gansu University of Chinese Medicine,Lanzhou 730000,China)

机构地区：[1]甘肃中医药大学药学院,甘肃兰州730000

出　　处：《中国中医药信息杂志》2022年第5期92-97,共6页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81860788);兰州市人才创新创业项目(2019-RC-101);陇西县星火科技计划项目(202001)。

摘　　要：目的优选金蕾颗粒水提醇沉工艺。方法采用L_(9)(3^(4))正交试验法,以料液比、提取时间、提取次数为考察因素,以当药黄素、木犀草素、山柰酚的提取量及干膏得率为考察指标,采用熵权法进行多指标综合加权评分,优选水提工艺;采用单因素试验,以药液密度、乙醇浓度、静置温度为考察因素,以当药黄素、木犀草素、山柰酚的转移率及干膏得率为考察指标,结合大生产实际情况优选醇沉工艺。结果优化的水提工艺为:加12倍量水,煎煮提取3次,每次0.5 h;优化的醇沉工艺为:药液密度1.03~1.05 g/mL,加入95%乙醇使含醇量达70%,10℃静置24 h。结论本研究优选的水提醇沉工艺稳定可靠、重复性好,可为金蕾颗粒的后续研究提供参考。Objective To optimize the water extraction and alcohol precipitation process of Jinlei Granules.Methods L_(9)(3^(4))orthogonal test was used for water extraction process.The ratio of material to liquid,extraction time and times of extraction were taken as the factors,and the extraction amount of swertisin,luteolin and kaempferol and the yield of dry extract were taken as the indexes.Entropy weight method was used for multi-index weighted scoring to optimize the water extraction process.The single factor method was used,with the extract density,ethanol volume fraction and the setting temperature as the factors,and the transfer rate of swertisin,luteolin and kaempferol as the index components,and the dry extract yield as the index.At the same time,the alcohol precipitation process was optimized according to the actual situation of large-scale production.Results The optimal extraction process was to add 12 times of water and decoct for 3 times,0.5 h for each extraction.The optimal alcohol precipitation process was as follows:the density of the liquid was 1.03–1.05 g/mL,95%ethanol was added to make the alcohol content up to 70%,and the liquid was left standing at 10℃for 24 h.Conclusion The optimized process of water extraction and alcohol precipitation is stable,reliable and with good repeatability,which can provide reference for the follow-up study of Jinlei Granules.

关 键 词：金蕾颗粒 水提醇沉 正交试验 熵权法 多指标综合评分法 

分 类 号：R283.5[医药卫生—中药学]